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RIAM Monoclonal antibody, PBS Only

RIAM Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F8A2

Cat no : 67143-1-PBS

Print datasheet

Synonyms

APBB1 interacting protein 1, APBB1IP, APBB1IP,RIAM, INAG1, PREL 1, PREL1, Proline rich EVH1 ligand 1, Proline rich protein 73, RARP 1, RARP1, RIAM, RIAM,APBB1IP

验证数据展示

  • WB analysis using 67143-1-Ig (same clone as 67143-1-PBS)

    WB analysis using 67143-1-Ig (same clone as 67143-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 67143-1-Ig (same clone as 67143-1-PBS)

    IHC staining of human tonsillitis using 67143-1-Ig (same clone as 67143-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 67143-1-Ig (same clone as 67143-1-PBS)

    IHC staining of human tonsillitis using 67143-1-Ig (same clone as 67143-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 67143-1-Ig (same clone as 67143-1-PBS)

    IF Staining of human tonsillitis using 67143-1-Ig (same clone as 67143-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using RIAM,APBB1IP antibody (67143-1-Ig, Clone: 2F8A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

产品信息

67143-1-PBS targets RIAM in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen RIAM fusion protein Ag4602 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein
Synonyms APBB1 interacting protein 1, APBB1IP, APBB1IP,RIAM, INAG1, PREL 1, PREL1, Proline rich EVH1 ligand 1, Proline rich protein 73, RARP 1, RARP1, RIAM, RIAM,APBB1IP
Calculated Molecular Weight 666 aa, 73 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC035636
Gene Symbol RIAM
Gene ID (NCBI) 54518
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7Z5R6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

APBB1IP gene encodes a Rap1-GTP-interacting adapter molecule (RIAM) which is an important protein in Rap1-mediated integrin activation and adhesion. Rap1 is a small GTPase frequently activated in tumors such as melanoma and prostate cancer. RIAM is widely expressed with high expression in thymus, spleen, lymph node, bone marrow and peripheral leukocytes. RIAM may function in the signal transduction from Ras activation to actin cytoskeletal remodeling. The observed molecular weight of RIAM is about 100 kDa.