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Species Reactivity
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验证数据展示

  • WB analysis using 67143-1-Ig (same clone as 67143-1-PBS)

    WB analysis using 67143-1-Ig (same clone as 67143-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 67143-1-Ig (same clone as 67143-1-PBS)

    IHC staining of human tonsillitis using 67143-1-Ig (same clone as 67143-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 67143-1-Ig (same clone as 67143-1-PBS)

    IHC staining of human tonsillitis using 67143-1-Ig (same clone as 67143-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 67143-1-Ig (same clone as 67143-1-PBS)

    IF Staining of human tonsillitis using 67143-1-Ig (same clone as 67143-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using RIAM,APBB1IP antibody (67143-1-Ig, Clone: 2F8A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

RIAM Monoclonal antibody, PBS Only

RIAM Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67143-1-PBS

产品说明书

CloneNo. 2F8A2

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

APBB1 interacting protein 1, APBB1IP, APBB1IP,RIAM, INAG1, PREL 1, PREL1, Proline rich EVH1 ligand 1, Proline rich protein 73, RARP 1, RARP1, RIAM, RIAM,APBB1IP

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67143-1-PBS targets RIAM in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 Human, Mouse
免疫原 RIAM fusion protein Ag4602 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein
别名 APBB1 interacting protein 1, APBB1IP, APBB1IP,RIAM, INAG1, PREL 1, PREL1, Proline rich EVH1 ligand 1, Proline rich protein 73, RARP 1, RARP1, RIAM, RIAM,APBB1IP
计算分子量 666 aa, 73 kDa
观测分子量 100 kDa
GenBank蛋白编号BC035636
基因名称 RIAM
Gene ID (NCBI) 54518
RRIDAB_2882442
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ7Z5R6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

APBB1IP gene encodes a Rap1-GTP-interacting adapter molecule (RIAM) which is an important protein in Rap1-mediated integrin activation and adhesion. Rap1 is a small GTPase frequently activated in tumors such as melanoma and prostate cancer. RIAM is widely expressed with high expression in thymus, spleen, lymph node, bone marrow and peripheral leukocytes. RIAM may function in the signal transduction from Ras activation to actin cytoskeletal remodeling. The observed molecular weight of RIAM is about 100 kDa.