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验证数据展示

  • WB analysis using 11138-1-AP

    WB analysis using 11138-1-AP

    BxPC-3 cells and Jurkat cells were subjected to SDS PAGE followed by western blot with 11138-1-AP (RIC8A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 11138-1-AP

    IHC staining of human pancreas cancer using 11138-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11138-1-AP (RIC8A antibody) at dilution of 1:50 (under 10x lens).

RIC8A Polyclonal antibody

RIC8A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11138-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Protein Ric 8A, RIC8, RIC8A, synembryn, Synembryn A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, human brain tissue, Jurkat cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11138-1-AP targets RIC8A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen RIC8A fusion protein Ag1593 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)
Synonyms Protein Ric 8A, RIC8, RIC8A, synembryn, Synembryn A
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 58-62 kDa
GenBank Accession NumberBC011821
Gene Symbol RIC8A
Gene ID (NCBI) 60626
RRIDAB_2179822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NPQ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.

实验方案

Product Specific Protocols
WB protocol for RIC8A antibody 11138-1-APDownload protocol
IHC protocol for RIC8A antibody 11138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

CYLD regulates spindle orientation by stabilizing astral microtubules and promoting dishevelled-NuMA-dynein/dynactin complex formation.

Authors - Yunfan Yang
View Article
mouseWB,IHC

Front Cell Neurosci

Ric-8A, a Galpha protein guanine nucleotide exchange factor potentiates taste receptor signaling.

Authors - Fenech Claire C
View Article