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RIC8B Polyclonal antibody

RIC8B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17790-1-AP

Print datasheet

Synonyms

Synembryn-B, RIC8, Protein Ric-8B, hSyn, Brain synembryn

验证数据展示

  • WB analysis of human brain using 17790-1-AP

    WB analysis of human brain using 17790-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17790-1-AP (RIC8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 17790-1-AP

    IHC staining of human brain using 17790-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17790-1-AP (RIC8B antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 17790-1-AP

    IHC staining of human brain using 17790-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17790-1-AP (RIC8B antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of MCF-7 using 17790-1-AP

    IF Staining of MCF-7 using 17790-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 17790-1-AP (RIC8B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17790-1-AP targets RIC8B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen RIC8B fusion protein Ag11888 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name resistance to inhibitors of cholinesterase 8 homolog B (C. elegans)
Synonyms Synembryn-B, RIC8, Protein Ric-8B, hSyn, Brain synembryn
Calculated Molecular Weight 495 aa, 56 kDa
Observed Molecular Weight 61-66 kDa
GenBank Accession NumberBC034220
Gene Symbol RIC8B
Gene ID (NCBI) 55188
RRIDAB_2285188
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVN3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ric8, also known as synembryn, is a guanine nucleotide exchange factor (GEF) that enhances variegated G protein signaling pathways, including Gα(q) and Gα(s) regulation of neurotransmission, Gα(i)-dependent mitotic spindle positioning during (asymmetric) cell division, and Gα(olf)-dependent odorant receptor signaling (PMID: 21467038). In mammals, two Ric8 homologues have been reported (Ric8A and Ric8B). Ric8B is able to potentiate G(s)/G(olf) signaling (PMID: 15829631; 16754875; 20133939).

实验方案

Product Specific Protocols
WB protocol for RIC8B antibody 17790-1-APDownload protocol
IHC protocol for RIC8B antibody 17790-1-APDownload protocol
IF protocol for RIC8B antibody 17790-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Front Cell Neurosci

Ric-8A, a Galpha protein guanine nucleotide exchange factor potentiates taste receptor signaling.

Authors - Fenech Claire C
View Article
mouseWB

Sci Rep

The BEACH Protein LRBA Promotes the Localization of the Heterotrimeric G-protein Golf to Olfactory Cilia.

Authors - Stefan Kurtenbach
View Article
mouseIF

PLoS One

Expression Pattern and Localization Dynamics of Guanine Nucleotide Exchange Factor RIC8 during Mouse Oogenesis.

Authors - Merly Saare
View Article