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RIG-1/DDX58 Monoclonal antibody, PBS Only

RIG-1/DDX58 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B10F5

Cat no : 67556-1-PBS

Print datasheet

Synonyms

DDX58, RIG-I, EC:3.6.4.13, DEAD box protein 58, ATP-dependent RNA helicase DDX58

验证数据展示

  • IF Staining of HepG2 using 67556-1-Ig (same clone as 67556-1-PBS)

    IF Staining of HepG2 using 67556-1-Ig (same clone as 67556-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RIG-1/DDX58 antibody (67556-1-Ig, Clone: 3B10F5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67556-1-PBS in a different storage buffer formulation.

  • WB analysis using 67556-1-Ig (same clone as 67556-1-PBS)

    WB analysis using 67556-1-Ig (same clone as 67556-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67556-1-Ig (DDX58 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67556-1-PBS in a different storage buffer formulation.

产品信息

67556-1-PBS targets RIG-1/DDX58 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RIG-1/DDX58 fusion protein Ag18591 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
Synonyms DDX58, RIG-I, EC:3.6.4.13, DEAD box protein 58, ATP-dependent RNA helicase DDX58
Calculated Molecular Weight 925 aa, 106 kDa
Observed Molecular Weight 101/106 kDa
GenBank Accession NumberBC132786
Gene Symbol RIG-1/DDX58
Gene ID (NCBI) 23586
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95786
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58,which has two isoform with the MW of 106 and 101 kDa.