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  • KD/KO Validated

RILP Polyclonal antibody

RILP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13574-1-AP

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Synonyms

Rab-interacting lysosomal protein, PP10141, FLJ31193



经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13574-1-AP targets RILP in WB, IHC, IF/ICC, ELISA, PLA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, PLA
Tested Reactivity human
Cited Reactivityhuman
Immunogen RILP fusion protein Ag4472 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rab interacting lysosomal protein
Synonyms Rab-interacting lysosomal protein, PP10141, FLJ31193
Calculated Molecular Weight 401 aa, 41 kDa
Observed Molecular Weight 41-45 kDa
GenBank Accession NumberBC031621
Gene Symbol RILP
Gene ID (NCBI) 83547
RRIDAB_2877959
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96NA2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RILP antibody 13574-1-APDownload protocol
IHC protocol for RILP antibody 13574-1-APDownload protocol
IF protocol for RILP antibody 13574-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy

Authors - Rahul Kumar
humanPLA

J Extracell Vesicles

Neddylation of Coro1a determines the fate of multivesicular bodies and biogenesis of extracellular vesicles.

Authors - Xuefeng Fei
humanWB

J Exp Clin Cancer Res

Temozolomide-Perillyl alcohol conjugate impairs Mitophagy flux by inducing lysosomal dysfunction in non-small cell lung Cancer cells and sensitizes them to irradiation.

Authors - Minghui Chang
humanWB

J Biol Chem

A comprehensive analysis of Rab GTPases reveals a role for Rab34 in serum starvation-induced primary ciliogenesis.

Authors - Mai E Oguchi
  • KD Validated
WB

Cell Death Dis

TRIM39 deficiency inhibits tumor progression and autophagic flux in colorectal cancer via suppressing the activity of Rab7.

Authors - Jia Hu
humanWB

Signal Transduct Target Ther

Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1.

Authors - Wenchang Xiao