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  • Featured Product
  • KD/KO Validated

RIMBP2 Polyclonal antibody

RIMBP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15716-1-AP

Print datasheet

Synonyms

KIAA0318, RBP2, RIM BP2, RIMBP2, RIMS binding protein 2

验证数据展示

  • WB analysis using 15716-1-AP

    WB analysis using 15716-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15716-1-AP (RIMBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 15716-1-AP

    IHC staining of mouse brain using 15716-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15716-1-AP (RIMBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15716-1-AP targets RIMBP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen RIMBP2 fusion protein Ag8390 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RIMS binding protein 2
Synonyms KIAA0318, RBP2, RIM BP2, RIMBP2, RIMS binding protein 2
Calculated Molecular Weight 116 kDa
Observed Molecular Weight 120-150 kDa
GenBank Accession NumberBC007632
Gene Symbol RIMBP2
Gene ID (NCBI) 23504
RRIDAB_2878173
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15034
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The presynaptic active zone mediates synaptic vesicle exocytosis at a synapse. RIM proteins are active zone scaffolding molecules that--among others--mediate vesicle priming and directly or indirectly interact with most other essential presynaptic proteins. RIMBP2 encodes RIMS-binding protein 2 belonging to RIMBP family. RIMBP2 is supposed to be localized at cell membrane especially synaptic plasma membrane. By interacting with RIMS1, RIMS2, CACNA1D and CACNA1B via the SH3 domains, RIMBP2 mediates synaptic transmission as bifunctional linker.

实验方案

Product Specific Protocols
WB protocol for RIMBP2 antibody 15716-1-APDownload protocol
IHC protocol for RIMBP2 antibody 15716-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Elife

Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa.

Authors - Fushun Fan
  • KD Validated
View Article
mouseWB

Elife

Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis

Authors - Kunli Zhao
View Article