验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
产品信息
15716-1-AP targets RIMBP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | RIMBP2 fusion protein Ag8390 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RIMS binding protein 2 |
| 别名 | KIAA0318, RBP2, RIM BP2, RIMBP2, RIMS binding protein 2 |
| 计算分子量 | 116 kDa |
| 观测分子量 | 120-150 kDa |
| GenBank蛋白编号 | BC007632 |
| 基因名称 | RIMBP2 |
| Gene ID (NCBI) | 23504 |
| RRID | AB_2878173 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15034 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The presynaptic active zone mediates synaptic vesicle exocytosis at a synapse. RIM proteins are active zone scaffolding molecules that--among others--mediate vesicle priming and directly or indirectly interact with most other essential presynaptic proteins. RIMBP2 encodes RIMS-binding protein 2 belonging to RIMBP family. RIMBP2 is supposed to be localized at cell membrane especially synaptic plasma membrane. By interacting with RIMS1, RIMS2, CACNA1D and CACNA1B via the SH3 domains, RIMBP2 mediates synaptic transmission as bifunctional linker.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RIMBP2 antibody 15716-1-AP | Download protocol |
| IHC protocol for RIMBP2 antibody 15716-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa.
| ||
Elife Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis | ||
Cancer Gene Ther N6-methyladenosine modification of RIMS binding protein 2 promotes head and neck squamous cell carcinoma proliferation and radiotherapy tolerance through endoplasmic reticulum stress |