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RIM2-Specific Polyclonal antibody

RIM2-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20093-1-AP

Print datasheet

Synonyms

KIAA0751, OBOE, Rab 3 interacting molecule 2, Rab 3 interacting protein 3, RAB3IP3, RIM 2, RIM2, RIM2-Specific, RIMS2

验证数据展示

  • WB analysis of L02 using 20093-1-AP

    WB analysis of L02 using 20093-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 20093-1-AP (RIM2-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 20093-1-AP

    IHC staining of mouse brain using 20093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20093-1-AP (RIM2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 20093-1-AP

    IHC staining of mouse brain using 20093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20093-1-AP (RIM2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inL02 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20093-1-AP targets RIM2-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name regulating synaptic membrane exocytosis 2
Synonyms KIAA0751, OBOE, Rab 3 interacting molecule 2, Rab 3 interacting protein 3, RAB3IP3, RIM 2, RIM2, RIM2-Specific, RIMS2
Calculated Molecular Weight 160 kDa
Observed Molecular Weight 133 kDa
GenBank Accession NumberNM_001100117
Gene Symbol RIMS2
Gene ID (NCBI) 9699
RRIDAB_10666429
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQ26
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RIMS2, also named as KIAA0751, RAB3IP3 and RIM2, is a rab effector which involved in exocytosis. RIMS2 may act as scaffold protein. This antibody is specific to RIMS2. It recognizes all the isoforms except isoform7 of RIMS2.

实验方案

Product Specific Protocols
WB protocol for RIM2-Specific antibody 20093-1-APDownload protocol
IHC protocol for RIM2-Specific antibody 20093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols