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验证数据展示

  • WB analysis of L02 using 20093-1-AP

    WB analysis of L02 using 20093-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 20093-1-AP (RIM2-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 20093-1-AP

    IHC staining of mouse brain using 20093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20093-1-AP (RIM2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 20093-1-AP

    IHC staining of mouse brain using 20093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20093-1-AP (RIM2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RIM2-Specific Polyclonal antibody

RIM2-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20093-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

KIAA0751, OBOE, Rab 3 interacting molecule 2, Rab 3 interacting protein 3, RAB3IP3, RIM 2, RIM2, RIM2-Specific, RIMS2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20093-1-AP targets RIM2-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 regulating synaptic membrane exocytosis 2
别名 KIAA0751, OBOE, Rab 3 interacting molecule 2, Rab 3 interacting protein 3, RAB3IP3, RIM 2, RIM2, RIM2-Specific, RIMS2
计算分子量 160 kDa
观测分子量 133 kDa
GenBank蛋白编号NM_001100117
基因名称 RIMS2
Gene ID (NCBI) 9699
RRIDAB_10666429
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UQ26
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RIMS2, also named as KIAA0751, RAB3IP3 and RIM2, is a rab effector which involved in exocytosis. RIMS2 may act as scaffold protein. This antibody is specific to RIMS2. It recognizes all the isoforms except isoform7 of RIMS2.

实验方案

Product Specific Protocols
WB protocol for RIM2-Specific antibody 20093-1-APDownload protocol
IHC protocol for RIM2-Specific antibody 20093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols