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验证数据展示

  • WB analysis of mouse skeletal muscle using 15037-1-AP

    WB analysis of mouse skeletal muscle using 15037-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15037-1-AP (RING1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 15037-1-AP

    IHC staining of human prostate cancer using 15037-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15037-1-AP (RING1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human prostate cancer using 15037-1-AP

    IHC staining of human prostate cancer using 15037-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15037-1-AP (RING1 antibody) at dilution of 1:50 (under 40x lens).

RING1 Polyclonal antibody

RING1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15037-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Polycomb complex protein RING1, ring finger protein 1, RING1, RING1A, RNF1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, BxPC-3 cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15037-1-AP targets RING1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RING1 fusion protein Ag6763 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 1
别名 Polycomb complex protein RING1, ring finger protein 1, RING1, RING1A, RNF1
计算分子量 42 kDa
观测分子量 50 kDa
GenBank蛋白编号BC002922
基因名称 RING1
Gene ID (NCBI) 6015
RRIDAB_2177964
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ06587
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ring1, also named as Ring1a, constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It's an essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.

实验方案

Product Specific Protocols
WB protocol for RING1 antibody 15037-1-APDownload protocol
IHC protocol for RING1 antibody 15037-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols