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RING1 Polyclonal antibody

RING1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15037-1-AP

Print datasheet

Synonyms

Polycomb complex protein RING1, ring finger protein 1, RING1, RING1A, RNF1

验证数据展示

  • WB analysis of mouse skeletal muscle using 15037-1-AP

    WB analysis of mouse skeletal muscle using 15037-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15037-1-AP (RING1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 15037-1-AP

    IHC staining of human prostate cancer using 15037-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15037-1-AP (RING1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human prostate cancer using 15037-1-AP

    IHC staining of human prostate cancer using 15037-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15037-1-AP (RING1 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, BxPC-3 cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15037-1-AP targets RING1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RING1 fusion protein Ag6763 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 1
Synonyms Polycomb complex protein RING1, ring finger protein 1, RING1, RING1A, RNF1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC002922
Gene Symbol RING1
Gene ID (NCBI) 6015
RRIDAB_2177964
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ06587
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ring1, also named as Ring1a, constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It's an essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.

实验方案

Product Specific Protocols
WB protocol for RING1 antibody 15037-1-APDownload protocol
IHC protocol for RING1 antibody 15037-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols