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RINT1 Polyclonal antibody

RINT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14567-1-AP

Print datasheet

Synonyms

RINT-1, RINT 1, RAD50-interacting protein 1, HsRINT-1, HsRINT1

验证数据展示

  • WB analysis of HepG2 using 14567-1-AP

    WB analysis of HepG2 using 14567-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14567-1-AP (RINT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 14567-1-AP

    IHC staining of human breast cancer using 14567-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14567-1-AP (RINT1 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 14567-1-AP

    IF Staining of HepG2 using 14567-1-AP

    Immunofluorescent analysis of HepG2 cells using 14567-1-AP (RINT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

14567-1-AP targets RINT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RINT1 fusion protein Ag6145 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAD50 interactor 1
Synonyms RINT-1, RINT 1, RAD50-interacting protein 1, HsRINT-1, HsRINT1
Calculated Molecular Weight 91 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC068483
Gene Symbol RINT1
Gene ID (NCBI) 60561
RRIDAB_2269421
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6NUQ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RINT-1 was first identified as a Rad50-interacting protein that participates in radiation-induced G2/M checkpoint control. It also involved in regulation of membrane traffic between the endoplasmic reticulum and the Golgi. It interacts with p130, an Rb family protein, and together they regulate the telomerase-independent telomere length. Otherwise, it interacts with Zeste white 10 with its N-terminal region throughout the cell cycle.

实验方案

Product Specific Protocols
WB protocol for RINT1 antibody 14567-1-APDownload protocol
IHC protocol for RINT1 antibody 14567-1-APDownload protocol
IF protocol for RINT1 antibody 14567-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Biol Chem

Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis

Authors - Robert S Kiss
View Article