验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
17222-1-AP targets RIOK1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | RIOK1 fusion protein Ag11057 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RIO kinase 1 (yeast) |
| 别名 | AD034, EC:2.7.11.1, RIO kinase 1, RIO1, Serine/threonine-protein kinase RIO1 |
| 计算分子量 | 568 aa, 66 kDa |
| 观测分子量 | 75-80 kDa |
| GenBank蛋白编号 | BC006104 |
| 基因名称 | RIOK1 |
| Gene ID (NCBI) | 83732 |
| RRID | AB_2284990 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BRS2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RIOK1 (RIO Kinase 1) is a protein-coding gene located on chromosome 6 in humans. It encodes a serine/threonine-protein kinase that belongs to the RIO family of atypical protein kinases/ATPases. This family also includes RIOK2, RIOK3, and RIOB, which have evolved from Archaea to humans. RIOK1 is best known for its role in ribosome biogenesis and its involvement in various cellular processes, including translation regulation and cell-cycle progression. (PMID: 37301535, PMID: 37935656)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RIOK1 antibody 17222-1-AP | Download protocol |
| IP protocol for RIOK1 antibody 17222-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomedicines RIOK2 Inhibitor NSC139021 Exerts Anti-Tumor Effects on Glioblastoma via Inducing Skp2-Mediated Cell Cycle Arrest and Apoptosis. | ||
iScience Ras Post-transcriptionally Enhances a Pre-malignantly Primed EMT to Promote Invasion. | ||
Cancer Res Treat Elevated Expression of RIOK1 Is Correlated with Breast Cancer Hormone Receptor Status and Promotes Cancer Progression. |