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验证数据展示

  • WB analysis using 17410-1-AP

    WB analysis using 17410-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17410-1-AP (RIOK2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 17410-1-AP

    IF Staining of U2OS using 17410-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using RIOK2 antibody (17410-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

RIOK2 Polyclonal antibody

RIOK2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17410-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Serine/threonine-protein kinase RIO2, RIO2, RIO kinase 2, EC:2.7.11.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCaco-2 cells, HCT 116 cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17410-1-AP targets RIOK2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 RIOK2 fusion protein Ag9975 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RIO kinase 2 (yeast)
别名 Serine/threonine-protein kinase RIO2, RIO2, RIO kinase 2, EC:2.7.11.1
计算分子量 63 kDa
观测分子量63 kDa
GenBank蛋白编号BC000953
基因名称 RIOK2
Gene ID (NCBI) 55781
RRIDAB_2178093
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BVS4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rio-kinase 2 (RIOK2) is an atypical serine-threonine kinase that participates in the nuclear export and maturation steps of the pre-40S ribosomal complex to facilitate cytoplasmic translation. RIOK2 binds the pre-40S subunit and promotes its nuclear export by interaction with the CRM1 chaperone protein (PMID: 36661680, 28499923).

实验方案

Product Specific Protocols
WB protocol for RIOK2 antibody 17410-1-APDownload protocol
IF protocol for RIOK2 antibody 17410-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols