验证数据展示
经过测试的应用
| Positive WB detected in | TNF-alpha treated HT-29 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28252-1-AP targets Phospho-RIPK1 (Ser166) in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | receptor (TNFRSF)-interacting serine-threonine kinase 1 |
| Synonyms | Cell death protein RIP, FLJ39204, Phospho-RIPK1 (Ser166), Receptor interacting protein 1, RIP, RIP 1, RIP1, RIPK1 |
| Calculated Molecular Weight | 76 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | NM_003804 |
| Gene Symbol | RIPK1 |
| Gene ID (NCBI) | 8737 |
| RRID | AB_2881098 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13546 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RIPK1, a 74 kDa protein, is composed of a N-terminal kinase domain, an intermediate domain (containing the RIP homotypic interaction motif, RHIM) and a C-terminal death domain. Stimulation of cells with TNFα can promote distinct cell death pathways, including RIPK1-independent apoptosis, necroptosis, and RIPK1-dependent apoptosis (RDA). TNFα induces cell necroptosis and the phosphorylation of RIPK1 at the Ser166 residue i.e. p-RIPK1 (Ser166), both of which can be effectively inhibited by Nec-1. Therefore, p-RIPK1 (Ser166) is considered a biomarker for the activation of RIPK1 kinase and necroptosis (PMID: 31440386, PMID: 29891719).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-RIPK1 (Ser166) antibody 28252-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Necroptosis enhances 'don't eat me' signal and induces macrophage extracellular traps to promote pancreatic cancer liver metastasis | ||
Int Immunopharmacol EGFR-TNFR1 pathway in endothelial cell facilitates acute lung injury by NF-κB/MAPK-mediated inflammation and RIP3-dependent necroptosis | ||
Cell Death Discov LncRNA HABON promoted liver cancer cells survival under hypoxia by inhibiting mPTP opening. | ||
Int J Biol Macromol Etelcalcetide ameliorates bone loss in chronic kidney disease-mineral and bone disorder by activation of IRF7 and necroptosis pathways | ||
J Cancer Jolkinolide B Inhibits Gastric Cancer Growth by Targeting the PANoptosis Molecular Switch Caspase-8 | ||
Inflamm Bowel Dis Phosphoproteomics Reveals Novel Insights into the Pathogenesis and Identifies New Therapeutic Kinase Targets of Ulcerative Colitis |