验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, THP-1 cells, HeLa cells |
| Positive IP detected in | HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15366-1-AP targets RIPK2 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 | RIPK2 fusion protein Ag0909 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | receptor-interacting serine-threonine kinase 2 |
| 别名 | EC:2.7.10.2, CCK, CARDIAK, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, CARD-containing IL-1 beta ICE-kinase |
| 计算分子量 | 61 kDa |
| 观测分子量 | 46-55 kDa, 61-72 kDa |
| GenBank蛋白编号 | BC004553 |
| 基因名称 | RIPK2 |
| Gene ID (NCBI) | 8767 |
| RRID | AB_2253650 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43353 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RIPK2 is also named as CARDIAK, RICK, RIP2 and belongs to the receptor-interacting serine/threonine-protein kinase(RIPK) family. RIPK2 functions as a key signaling protein in host defense responses induced by activation of the cytosolic pattern recognition receptors (PRR) NOD1 and NOD2. It has also been reported to mediate other cellular responses, including TLR signaling, caspase 1 activation , apoptosis and autophagy(PMID:22685397). It has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RIPK2 antibody 15366-1-AP | Download protocol |
| IP protocol for RIPK2 antibody 15366-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Antioxidants (Basel) M6A RNA Methylation Mediates NOD1/NF-kB Signaling Activation in the Liver of Piglets Challenged with Lipopolysaccharide | ||
Obesity (Silver Spring) Suppression of RIP1 activity via S415 dephosphorylation ameliorates obesity-related hepatic insulin resistance. | ||
J Pharmacol Exp Ther Identification and Characterization of Novel Receptor-Interacting Serine/Threonine-Protein Kinase 2 Inhibitors Using Structural Similarity Analysis.
| ||
Front Cell Infect Microbiol Extracellular Histone H3 Induces Pyroptosis During Sepsis and May Act Through NOD2 and VSIG4/NLRP3 Pathways. | ||
Immunol Lett A small molecule inhibitor of caspase-1 inhibits NLRP3 inflammasome activation and pyroptosis to alleviate gouty inflammation. | ||
Front Genet Knockdown of RIPK2 Inhibits Proliferation and Migration, and Induces Apoptosis via the NF-κB Signaling Pathway in Gastric Cancer.
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