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RIPK3 Polyclonal antibody

RIPK3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29080-1-AP

Print datasheet

Synonyms

RIP3, RIP like protein kinase 3, RIP 3, Receptor-interacting serine/threonine-protein kinase 3, Receptor-interacting protein 3

验证数据展示

  • WB analysis using 29080-1-AP

    WB analysis using 29080-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 29080-1-AP (RIP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inTHP-1 cells
Planning an IHC experiment? We recommend our IHCeasy RIPK3 Ready-To-Use IHC Kit. RIPK3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29080-1-AP targets RIPK3 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen RIPK3 fusion protein Ag30337 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name receptor-interacting serine-threonine kinase 3
Synonyms RIP3, RIP like protein kinase 3, RIP 3, Receptor-interacting serine/threonine-protein kinase 3, Receptor-interacting protein 3
Calculated Molecular Weight 518 aa, 57 kDa
Observed Molecular Weight57 kDa
GenBank Accession NumberBC062584
Gene Symbol RIPK3
Gene ID (NCBI) 11035
RRIDAB_3086097
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y572
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RIPK3,also named as RIP3, a Ser/Thr kinase of RIP (Receptor Interacting Protein) family, is recruited to the TNFR1 signaling complex through RIP and has been shown to mediate apoptosis induction and NF-κB activation. RIPK3 is a nucleocytoplasmic shuttling protein and its unconventional nuclear localization signal (NLS, 442-472 aa) is sufficient to trigger apoptosis in the nucleus(PMID:18533105). It has 3 isoforms produced by alternative splicing. RIPK3 might form a homodimer within cells, and its apoptotic activity may not be required for this dimerization(PMID:18533105).

实验方案

Product Specific Protocols
WB protocol for RIPK3 antibody 29080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols