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RIPK3 Monoclonal antibody, PBS Only (Detector)

RIPK3 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F7B3

Cat no : 68786-2-PBS

Print datasheet

Synonyms

RIP3, RIP 3, Receptor-interacting serine/threonine-protein kinase 3, Receptor-interacting protein 3, Receptor interacting protein 3

验证数据展示

  • WB analysis using 68786-2-Ig (same clone as 68786-2-PBS)

    WB analysis using 68786-2-Ig (same clone as 68786-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68786-2-Ig (RIPK3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68786-2-PBS in a different storage buffer formulation.

  • WB analysis of HT-29 using 68786-2-Ig (same clone as 68786-2-PBS)

    WB analysis of HT-29 using 68786-2-Ig (same clone as 68786-2-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 68786-2-Ig (RIPK3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68786-2-PBS in a different storage buffer formulation.

  • WB analysis of SK-BR-3 using 68786-2-Ig (same clone as 68786-2-PBS)

    WB analysis of SK-BR-3 using 68786-2-Ig (same clone as 68786-2-PBS)

    SK-BR-3 cells were subjected to SDS PAGE followed by western blot with 68786-2-Ig (RIPK3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68786-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50152-1

    Cytometric bead array standard curve of MP50152-1

    Cytometric bead array standard curve of MP50152-1, RIPK3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68786-1-PBS. Detection antibody: 68786-2-PBS. Standard:Ag11759. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP50152-3

    Cytometric bead array standard curve of MP50152-3

    Cytometric bead array standard curve of MP50152-3, RIPK3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68786-3-PBS. Detection antibody: 68786-2-PBS. Standard:Ag11759. Range: 0.391-50 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy RIPK3 Ready-To-Use IHC Kit. RIPK3 primary antibody included.

产品信息

68786-2-PBS targets RIPK3 as part of a matched antibody pair:

MP50152-1: 68786-1-PBS capture and 68786-2-PBS detection (validated in Cytometric bead array)

MP50152-3: 68786-3-PBS capture and 68786-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen RIPK3 fusion protein Ag11759 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name receptor-interacting serine-threonine kinase 3
Synonyms RIP3, RIP 3, Receptor-interacting serine/threonine-protein kinase 3, Receptor-interacting protein 3, Receptor interacting protein 3
Calculated Molecular Weight 518 aa, 57 kDa
Observed Molecular Weight57 kDa
GenBank Accession NumberBC062584
Gene Symbol RIPK3
Gene ID (NCBI) 11035
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y572
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Receptor-interacting protein 3 (RIP3, also known as RIPK3) is a serine-threonine protein involved in the regulation of inflammatory signaling and cell death. RIPK3, also named as RIP3, a Ser/Thr kinase of RIP (Receptor Interacting Protein) family, is a nucleocytoplasmic shuttling protein and its unconventional nuclear localization signal (NLS, 442-472 aa) is sufficient to trigger apoptosis in the nucleus (PMID: 18533105). It has 3 isoforms produced by alternative splicing.

What is the molecular weight of RIP3? Is RIP3 post-translationally modified?

The molecular weight of RIP3 is 57 kDa. During the induction of necroptosis, RIP3 migrates slower in SDS-PAGE due to its phosphorylation (PMID: 19524512). Additionally, RIP3 can be a subject of poly-ubiquitination, when targeted for degradation.

Are there any splice isoforms of RIP3?

Apart from full-length RIP3, there are two reported splice isoforms of RIP3: RIP3β and RIP3γ, and 28 and 25 kDa, respectively (PMID: 15896315).

What is the subcellular localization of RIP3?

RIP3 can shuttle between the cytoplasm and the nucleus. Although RIP3 forms necrosomes in the cytoplasm, a recent study suggests that the phosphorylation of RIP3, required for necroptosis, may also occur in the nucleus (PMID: 30271893). Additionally, the induction of necrosis by reactive oxygen species can cause transient translocation of RIP3 to mitochondria (PMID: 25206339).

What is the role of RIP3 in cell death (necroptosis)?

Activation of RIP3 kinase is required for the induction of necroptosis (PMID: 19524512, 19524513 and 19498109). Activation of RIP3 can be induced by interferons, death ligands, or by Toll-like receptors in response to pathogens. That leads to the phosphorylation of RIP3 and the formation of a β-amyloid-like protein complex. Phosphorylated RIP3 acts downstream by phosphorylation of MLKL (PMID: 30131615).

How to study necroptosis in a cell-based system?

The choice of the cell line is important. Many commonly used immortalized cell types are derived from cancers and may have very low RIL3 expression level (PMID: 25952668). Those cell lines are not going to be responsive to necrotic stimuli. A few of the examined cell types have high RIP3 levels: Jurkat, CCRF-CEM, U937, L929 cells, and mouse embryonic fibroblasts (PMID: 19524512). Good necroptosis readouts reflect an increased level of RIP3 protein and its phosphorylation.