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验证数据展示

  • IP experiment of SKOV-3 using 26458-1-AP

    IP experiment of SKOV-3 using 26458-1-AP

    IP result of anti-RIPK4 (IP:26458-1-AP, 4ug; Detection:26458-1-AP 1:300) with SKOV-3 cells lysate 2800ug.

  • IHC staining of human lung cancer using 26458-1-AP

    IHC staining of human lung cancer using 26458-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26458-1-AP (RIPK4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 26458-1-AP

    IHC staining of human lung cancer using 26458-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26458-1-AP (RIPK4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RIPK4 Polyclonal antibody

RIPK4 Polyclonal Antibody for IP, IHC, ELISA
Cat No. 26458-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IP, IHC, ELISA

别名

ANKK2, ANKRD3, DIK, PKK, Protein kinase, RIP4, RIPK4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inSKOV-3 cells
Positive IHC detected inhuman lung cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26458-1-AP targets RIPK4 in IP, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IP, IHC, ELISA Application Description
经测试反应性 human
免疫原 RIPK4 fusion protein Ag24202 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 receptor-interacting serine-threonine kinase 4
别名 ANKK2, ANKRD3, DIK, PKK, Protein kinase, RIP4, RIPK4
计算分子量 784 aa, 86 kDa
GenBank蛋白编号BC110617
基因名称 RIPK4
Gene ID (NCBI) 54101
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96T11
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.