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验证数据展示

  • IP experiment of SKOV-3 using 26458-1-AP

    IP experiment of SKOV-3 using 26458-1-AP

    IP result of anti-RIPK4 (IP:26458-1-AP, 4ug; Detection:26458-1-AP 1:300) with SKOV-3 cells lysate 2800ug.

  • IHC staining of human lung cancer using 26458-1-AP

    IHC staining of human lung cancer using 26458-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26458-1-AP (RIPK4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 26458-1-AP

    IHC staining of human lung cancer using 26458-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26458-1-AP (RIPK4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RIPK4 Polyclonal antibody

RIPK4 Polyclonal Antibody for IP, IHC, ELISA
Cat No. 26458-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, IHC, ELISA

Synonyms

ANKK2, ANKRD3, DIK, PKK, Protein kinase, RIP4, RIPK4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IP detected inSKOV-3 cells
Positive IHC detected inhuman lung cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26458-1-AP targets RIPK4 in IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IP, IHC, ELISA Application Description
Tested Reactivity human
Immunogen RIPK4 fusion protein Ag24202 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name receptor-interacting serine-threonine kinase 4
Synonyms ANKK2, ANKRD3, DIK, PKK, Protein kinase, RIP4, RIPK4
Calculated Molecular Weight 784 aa, 86 kDa
GenBank Accession NumberBC110617
Gene Symbol RIPK4
Gene ID (NCBI) 54101
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96T11
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.