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RIT2 Polyclonal antibody, PBS Only

RIT2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12473-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 12473-1-AP (same clone as 12473-1-PBS)

    WB analysis using 12473-1-AP (same clone as 12473-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12473-1-AP (RIT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12473-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 12473-1-AP (same clone as 12473-1-PBS)

    IP experiment of mouse brain using 12473-1-AP (same clone as 12473-1-PBS)

    IP result of anti-RIT2 (IP:12473-1-AP, 4ug; Detection:12473-1-AP 1:1000) with mouse brain tissue lysate 1800 ug. This data was developed using the same antibody clone with 12473-1-PBS in a different storage buffer formulation.

  • IP experiment of rat brain using 12473-1-AP (same clone as 12473-1-PBS)

    IP experiment of rat brain using 12473-1-AP (same clone as 12473-1-PBS)

    IP result of anti-RIT2 (IP:12473-1-AP, 4ug; Detection:12473-1-AP 1:1000) with rat brain tissue lysate 1120 ug. This data was developed using the same antibody clone with 12473-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 12473-1-AP (same clone as 12473-1-PBS)

    IHC staining of mouse brain using 12473-1-AP (same clone as 12473-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12473-1-AP (RIT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12473-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 12473-1-AP (same clone as 12473-1-PBS)

    IHC staining of mouse pancreas using 12473-1-AP (same clone as 12473-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12473-1-AP (RIT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12473-1-PBS in a different storage buffer formulation.

产品信息

12473-1-PBS targets RIT2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RIT2 fusion protein Ag3160 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Ras-like without CAAX 2
Synonyms
Calculated Molecular Weight 217 aa, 25 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC018060
Gene Symbol RIT2
Gene ID (NCBI) 6014
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99578
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RIT2 (AKA: Rin, Ras-like in neurons) is a small, neuronal, ras-like GTPase with enriched expression in SNc DANs (PMID: 38395968). RIT2 is a member of the Ras superfamily that plays important roles in many vital cellular functions, such as differentiation and survival (PMID: 29860660). Rit2 directly interacts with the DA transporter (DAT), and is required for regulated DAT membrane trafficking. In cell culture models, Rit2 is required for EGF- and NGF-mediated neurite outgrowth, NGF-mediated ERK phosphorylation, and cell viability (PMID: 38395968).