RLBP1 Polyclonal antibody

RLBP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 15356-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

Retinaldehyde-binding protein 1, CRALBP, Cellular retinaldehyde-binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse eye tissue, HepG2 cells, mouse retina tissue, rat eye tissue
Positive IHC detected inmouse eye tissue, human eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue, Retinal organoids
Positive IF/ICC detected inHeLa cells, Y79 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15356-1-AP targets RLBP1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, zebrafish
免疫原 RLBP1 fusion protein Ag7602 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 retinaldehyde binding protein 1
别名 Retinaldehyde-binding protein 1, CRALBP, Cellular retinaldehyde-binding protein
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC004199
基因名称 RLBP1
Gene ID (NCBI) 6017
RRIDAB_2178530
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP12271
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RLBP1 (Retinaldehyde-binding protein 1) is also named as cellular retinaldehyde-binding protein (CRALBP). CRALBP, a 36-kDa aqueous soluble carrier, is expressed in abundance by both retinal pigment epithelial (RPE) and Muller cells of retina and is important for rod- and cone-driven vision (PMID: 32188692). Retinal RLBP1 expression was decreased in diabetes, and its overexpression in Müller glia mitigated DR-associated neurovascular degeneration (PMID: 33674409).

实验方案

Product Specific Protocols
WB protocol for RLBP1 antibody 15356-1-APDownload protocol
IHC protocol for RLBP1 antibody 15356-1-APDownload protocol
IF protocol for RLBP1 antibody 15356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
zebrafishIF

Aging Cell

Müller Glia maintain their regenerative potential despite degeneration in the aged zebrafish retina.

Authors - Raquel R Martins
humanWB,IF

Mol Vis

Morphological, biochemical, and transcriptomic characterization of iPSC-derived human RPE cells from normal and Smith-Lemli-Opitz syndrome patients

Authors - Michael H Farkas
mouseIF

Diabetes

CD40 in Retinal Müller Cells Induces P2X7-Dependent Cytokine Expression in Macrophages/Microglia in Diabetic Mice and Development of Early Experimental Diabetic Retinopathy.

Authors - Jose-Andres C Portillo
mouseWB

FASEB J

A cell-penetrating CD40-TRAF2,3 blocking peptide diminishes inflammation and neuronal loss after ischemia/reperfusion.

Authors - Jose-Andres C Portillo
ratIF

iScience

Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration

Authors - Haibin Tian
humanIF

Invest Ophthalmol Vis Sci

CD40 Upregulation in the Retina of Patients With Diabetic Retinopathy: Association With TRAF2/TRAF6 Upregulation and Inflammatory Molecule Expression

Authors - Sarah Vos
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