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- KD/KO Validated
RLIM Polyclonal antibody
RLIM Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, Jurkat cells |
Positive IP detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16121-1-AP targets RLIM in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IP, ELISA Application Description |
Cited Applications | WB, IP, IF, IHC, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | RLIM fusion protein Ag9077 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ring finger protein, LIM domain interacting |
Synonyms | NY REN 43, R LIM, RING finger protein 12, RLIM, RNF12 |
Calculated Molecular Weight | 624 aa, 69 kDa |
Observed Molecular Weight | 75 kDa |
GenBank Accession Number | BC013357 |
Gene Symbol | RLIM |
Gene ID (NCBI) | 51132 |
RRID | AB_2181967 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NVW2 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for RLIM antibody 16121-1-AP | Download protocol |
IP protocol for RLIM antibody 16121-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Death Dis RNF12 is regulated by AKT phosphorylation and promotes TGF-β driven breast cancer metastasis. | ||
Oncotarget PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1. | ||
J Mol Cell Biol Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression.
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