验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14630-1-AP targets RMI1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | RMI1 fusion protein Ag6208 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae) |
| 别名 | BLAP75, C9orf76, FAAP75, RMI1, RP11 346I8.1 |
| 计算分子量 | 70 kDa |
| 观测分子量 | 75 kDa |
| GenBank蛋白编号 | BC064937 |
| 基因名称 | RMI1 |
| Gene ID (NCBI) | 80010 |
| RRID | AB_2301203 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H9A7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RMI1 is an essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. RMI1 promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. RMI1 is required for BLM phosphorylation during mitosis. Within the BLM complex, RMI1 is required for BLM and TOP3A stability. (PMID: 16537486, PMID: 16595695, PMID: 15775963)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RMI1 antibody 14630-1-AP | Download protocol |
| IHC protocol for RMI1 antibody 14630-1-AP | Download protocol |
| IP protocol for RMI1 antibody 14630-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Topoisomerase 3α Is Required for Decatenation and Segregation of Human mtDNA.
| ||
Nat Commun Parallel genome-wide screens identify synthetic viable interactions between the BLM helicase complex and Fanconi anemia.
| ||
J Biol Chem DNA2 cooperates with the WRN and BLM RecQ helicases to mediate long-range DNA-end resection in human cells.
| ||
J Cancer Hsa_circ_0091581 promotes glioma progression by regulating RMI1 via sponging miR-1243-5p. | ||
DNA Cell Biol Identification and Bioinformatic Assessment of circRNA Expression After RMI1 Knockdown and Ionizing Radiation Exposure. | ||
Int J Mol Sci Accumulation and Phosphorylation of RecQ-Mediated Genome Instability Protein 1 (RMI1) at Serine 284 and Serine 292 during Mitosis. |