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  • Featured Product
  • KD/KO Validated

RMND5A Polyclonal antibody

RMND5A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17559-1-AP

Print datasheet

Synonyms

CTLH, p44CTLH, Protein RMD5 homolog A, RMD5, RMND5A

验证数据展示

  • WB analysis of human plasma using 17559-1-AP

    WB analysis of human plasma using 17559-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 17559-1-AP (RMND5A antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IHC staining of human breast cancer using 17559-1-AP

    IHC staining of human breast cancer using 17559-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RMND5A antibody included
  • IHC staining of human breast cancer using 17559-1-AP

    IHC staining of human breast cancer using 17559-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RMND5A antibody included

经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RMND5A Ready-To-Use IHC Kit. RMND5A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17559-1-AP targets RMND5A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen RMND5A fusion protein Ag11751 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name required for meiotic nuclear division 5 homolog A (S. cerevisiae)
Synonyms CTLH, p44CTLH, Protein RMD5 homolog A, RMD5, RMND5A
Calculated Molecular Weight 391 aa, 44 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC047668
Gene Symbol RMND5A
Gene ID (NCBI) 64795
RRIDAB_2878416
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H871
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RMND5A antibody 17559-1-APDownload protocol
IHC protocol for RMND5A antibody 17559-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancers (Basel)

Oncopeptide MBOP Encoded by LINC01234 Promotes Colorectal Cancer through MAPK Signaling Pathway.

Authors - Chunyuan Tang
  • KD Validated
View Article
humanWB

Chembiochem

Identifying E3 ligase substrates with quantitative degradation proteomics

Authors - Victoria N Jordan
View Article
humanWB

Mol Cell

mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway

Authors - Sang Ah Yi
  • KO Validated
View Article