验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lymphoma tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy C19orf2 Ready-To-Use IHC Kit. C19orf2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11277-1-AP targets RMP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP, IF, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | RMP fusion protein Ag1789 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 19 open reading frame 2 |
| 别名 | C19orf2, NNX3, PPP1R19, Protein NNX3, RMP, RPB5 mediating protein, URI |
| 计算分子量 | 65 kDa |
| 观测分子量 | 70-80 kDa |
| GenBank蛋白编号 | BC026184 |
| 基因名称 | RMP |
| Gene ID (NCBI) | 8725 |
| RRID | AB_10596929 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O94763 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RMP antibody 11277-1-AP | Download protocol |
| IHC protocol for RMP antibody 11277-1-AP | Download protocol |
| IP protocol for RMP antibody 11277-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers
| ||
Oncogene RMP promotes venous metastases of hepatocellular carcinoma through promoting IL-6 transcription.
| ||
Cell Death Dis URI regulates tumorigenicity and chemotherapeutic resistance of multiple myeloma by modulating IL-6 transcription.
| ||
Am J Cancer Res Silence of URI in gastric cancer cells promotes cisplatin-induced DNA damage and apoptosis
| ||
Cardiovasc Res Cardiomyocyte-specific loss of RMP causes myocardial dysfunction and heart failure. |
