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验证数据展示

  • WB analysis using 18172-1-AP

    WB analysis using 18172-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18172-1-AP (RNASE2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 18172-1-AP

    IHC staining of human colon using 18172-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18172-1-AP (RNASE2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RNASE2/EDN Polyclonal antibody

RNASE2/EDN Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18172-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

RNASE2, RNase UpI-2, RNase 2, Non-secretory ribonuclease, Eosinophil-derived neurotoxin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman urine sample
Positive IHC detected inhuman normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18172-1-AP targets RNASE2/EDN in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen RNASE2/EDN fusion protein Ag12834 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin)
Synonyms RNASE2, RNase UpI-2, RNase 2, Non-secretory ribonuclease, Eosinophil-derived neurotoxin
Calculated Molecular Weight 161 aa, 18 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberBC093678
Gene Symbol RNASE2
Gene ID (NCBI) 6036
RRIDAB_3669293
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10153
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RNASE2/EDN antibody 18172-1-APDownload protocol
IHC protocol for RNASE2/EDN antibody 18172-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols