验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 2 publications below |
| IF | See 2 publications below |
产品信息
55338-1-AP targets RNASE3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribonuclease, RNase A family, 3 (eosinophil cationic protein) |
| 别名 | ECP, Eosinophil cationic protein, Ribonuclease 3, RNase 3, RNASE3, RNS3 |
| 计算分子量 | 18 kDa |
| 观测分子量 | 18 kDa |
| GenBank蛋白编号 | NM_002935 |
| 基因名称 | RNASE3 |
| Gene ID (NCBI) | 6037 |
| RRID | AB_2881312 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P12724 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNASE3 antibody 55338-1-AP | Download protocol |
| IHC protocol for RNASE3 antibody 55338-1-AP | Download protocol |
| IF protocol for RNASE3 antibody 55338-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Allergy Immunoglobulin G4 in eosinophilic esophagitis: Immune complex formation and correlation with disease activity | ||
Cell Commun Signal Allergen-induced CD11c + dendritic cell pyroptosis aggravates allergic rhinitis | ||
J Pharm Biomed Anal Network pharmacology analysis and experimental validation of Xiao-Qing-Long-Tang's therapeutic effects against neutrophilic asthma | ||
Int Immunopharmacol Androgens have therapeutic potential in T2 asthma by mediating METTL3 in bronchial epithelial cells |
