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验证数据展示

  • WB analysis using 30732-1-AP

    WB analysis using 30732-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30732-1-AP (RNASE4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 30732-1-AP

    WB analysis using 30732-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30732-1-AP (RNASE4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of rat lung using 30732-1-AP

    IHC staining of rat lung using 30732-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 30732-1-AP (RNASE4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat lung using 30732-1-AP

    IHC staining of rat lung using 30732-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 30732-1-AP (RNASE4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RNASE4 Polyclonal antibody

RNASE4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30732-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

Ribonuclease 4, RNase 4, RNASE4, RNS4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman milk, THP-1 cells
Positive IHC detected inrat lung tissue, mouse bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30732-1-AP targets RNASE4 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 RNASE4 fusion protein Ag29272 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribonuclease, RNase A family, 4
别名 Ribonuclease 4, RNase 4, RNASE4, RNS4
观测分子量17-20 kDa
GenBank蛋白编号BC015520
基因名称 RNASE4
Gene ID (NCBI) 6038
RRIDAB_3086405
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP34096
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human Ribonuclease 4 (RNase 4), one of the eight members of the human RNase A superfamily of endoribonucleases, is a uridine-specific endoribonuclease with a preference to cleave RNA after uridine residues prior to purines (PMID: 33818125). RNase 4 is a newly identified host defense peptide in the human kidney and bladder and kills uropathogenic E. coli (UPEC) and multi-drug resistant UPEC (PMID: 28955965).

实验方案

Product Specific Protocols
WB protocol for RNASE4 antibody 30732-1-APDownload protocol
IHC protocol for RNASE4 antibody 30732-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols