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RNASE6 Polyclonal antibody

RNASE6 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16425-1-AP

Print datasheet

Synonyms

RNS6, RNase K6, EC:3.1.27.-

验证数据展示

  • IHC staining of human liver using 16425-1-AP

    IHC staining of human liver using 16425-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16425-1-AP (RNASE6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 16425-1-AP

    IHC staining of mouse heart using 16425-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16425-1-AP (RNASE6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of THP-1 using 16425-1-AP

    IF Staining of THP-1 using 16425-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using RNASE6 antibody (16425-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive IHC detected inhuman liver tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16425-1-AP targets RNASE6 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen RNASE6 fusion protein Ag9667 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribonuclease, RNase A family, k6
Synonyms RNS6, RNase K6, EC:3.1.27.-
Calculated Molecular Weight 150 aa, 17 kDa
GenBank Accession NumberBC020848
Gene Symbol RNASE6
Gene ID (NCBI) 6039
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ93091
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rnase6, a protein belonging to the superfamily, is considered the only vertebrate specific enzyme known to have a wide range of physiological functions, including digestion, cytotoxicity, angiogenesis, male reproduction, and host defense. Rnase6 expression was upregulated in the peripheral blood and plaque tissues of AS patients (PMID: 34395402).

实验方案

Product Specific Protocols
IHC protocol for RNASE6 antibody 16425-1-APDownload protocol
IF protocol for RNASE6 antibody 16425-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols