验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human liver tissue, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
产品信息
16132-1-AP targets RNASEH2A in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | RNASEH2A fusion protein Ag9118 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribonuclease H2, subunit A |
| 别名 | AGS4, JUNB, Ribonuclease H2 subunit A, ribonuclease H2, subunit A, Ribonuclease HI large subunit, Ribonuclease HI subunit A, RNase H(35), RNase H2 subunit A, RNase HI large subunit, RNASEH2A, RNASEHI, RNHIA, RNHL |
| 计算分子量 | 299 aa, 33 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC011748 |
| 基因名称 | RNASEH2A |
| Gene ID (NCBI) | 10535 |
| RRID | AB_2269729 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75792 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNASEH2A(Ribonuclease H2 subunit A) is also named as RNASEHI, RNHIA and belongs to the RNase HII family and Eukaryotic subfamily. It is involved in the removal of the initiator RNA primers of Okazaki fragments, an essential step during chromosomal DNA replication of the lagging strand(PMID:9482870). It is an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNASEH2A antibody 16132-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Commun Biol RNaseH2A downregulation drives inflammatory gene expression via genomic DNA fragmentation in senescent and cancer cells | ||
PLoS Genet Aicardi-Goutières syndrome gene Rnaseh2c is a metastasis susceptibility gene in breast cancer.
| ||
Oncol Rep RNaseH2A is involved in human gliomagenesis through the regulation of cell proliferation and apoptosis. | ||
Tumori Hypoxia-induced RNASEH2A limits activation of cGAS-STING signaling in HCC and predicts poor prognosis. | ||
Cancer Res Commun Ribonuclease H2 Subunit A Preserves Genomic Integrity and Promotes Prostate Cancer Progression
| ||