RNASET2 Polyclonal antibody

RNASET2 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13753-1-AP

Print datasheet

Synonyms

RNase T2, RNASE6PL, Ribonuclease T2, Ribonuclease 6, EC:4.6.1.19



经过测试的应用

Positive IP detected inBxPC-3 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected intransfected cells
Planning an IHC experiment? We recommend our IHCeasy RNASET2 Ready-To-Use IHC Kit. RNASET2 primary antibody included.

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

13753-1-AP targets RNASET2 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen RNASET2 fusion protein Ag4695 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribonuclease T2
Synonyms RNase T2, RNASE6PL, Ribonuclease T2, Ribonuclease 6, EC:4.6.1.19
Calculated Molecular Weight 256 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC039713
Gene Symbol RNase T2
Gene ID (NCBI) 8635
RRIDAB_2285339
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00584
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNASET2(Ribonuclease T2) is also named as RNASE6PL(Ribonuclease 6) and belongs to the RNase T2 family. It is a unique member of the growing family of tumor-antagonizing genes and behaves as a class II tumor suppressor and abolish the tumorigenic potential of an ovarian cancer-derived cell line. This protein has 2 isoforms produced by alternative splicing. Both mildly glycosylated (ranging from38 to 45 kDa) and highly glycosylated forms (ranging from 50 to 80 kDa) of human tumor suppressor protein RNASET2 can be detected in immunoblotting in P. Pastoris(PMID: 21446958).

实验方案

Product Specific Protocols
IHC protocol for RNASET2 antibody 13753-1-APDownload protocol
IP protocol for RNASET2 antibody 13753-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

FEBS J

Identification of signal peptide features for substrate specificity in human Sec62/Sec63-dependent ER protein import.

Authors - Stefan Schorr
humanIHC

Cell Transplant

Development and Clinical Validation of a Seven-Gene Prognostic Signature Based on Multiple Machine Learning Algorithms in Kidney Cancer.

Authors - Mi Tian
mouse

Cell Signal

Musashi-2 binds with Fbxo6 to induce Rnaset2 ubiquitination and chemokine signaling pathway during vascular smooth muscle cell phenotypic switch in atherosclerosis

Authors - Tao Zhang
human

Arch Insect Biochem Physiol

Exploring the role of RNASET2 in the immune response of black soldier fly larvae

Authors - Sara Caramella
human

Nat Commun

PMID: 30842415

Authors - Juanjuan Ou