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  • Featured Product
  • KD/KO Validated

RNF10 Polyclonal antibody

RNF10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16936-1-AP

Print datasheet

Synonyms

RING finger protein 10, RIE2, KIAA0262, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF10

验证数据展示

  • WB analysis of PC-3 using 16936-1-AP

    WB analysis of PC-3 using 16936-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 16936-1-AP

    WB analysis using 16936-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-3 using 16936-1-AP

    IP experiment of PC-3 using 16936-1-AP

    IP result of anti-RNF10 (IP:16936-1-AP, 4ug; Detection:16936-1-AP 1:500) with PC-3 cells lysate 1480 ug.

  • IHC staining of human brain using 16936-1-AP

    IHC staining of human brain using 16936-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16936-1-AP (RNF10 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with RNF10 antibody included
  • IHC staining of human brain using 16936-1-AP

    IHC staining of human brain using 16936-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16936-1-AP (RNF10 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with RNF10 antibody included
  • IF Staining of HepG2 using 16936-1-AP

    IF Staining of HepG2 using 16936-1-AP

    Immunofluorescent analysis of HepG2 cells, using RNF10 antibody 16936-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inPC-3 cells, Jurkat cells, human brain tissue
Positive IP detected inPC-3 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RNF10 Ready-To-Use IHC Kit. RNF10 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16936-1-AP targets RNF10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen RNF10 fusion protein Ag10433 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 10
Synonyms RING finger protein 10, RIE2, KIAA0262, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF10
Calculated Molecular Weight 811 aa, 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC016622
Gene Symbol RNF10
Gene ID (NCBI) 9921
RRIDAB_2181846
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N5U6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RING finger (C3HC4-type zinc finger) is a variant zinc finger motif present in a new family of proteins including transcription regulators. RNF10 (RING finger protein 10), a 90-kDa protein, contains a ring finger motif which is known to be involved in protein-protein interactions. RNF10 regulates myelin-associated glycoprotein (MAG) expression and is required for myelin formation, therefore, participates in the peripheral nerve development and Schawnn cell differentiation. RNF10 is also involved in activation of p21WAF1 promoter via its activity as transcriptional factor.

实验方案

Product Specific Protocols
WB protocol for RNF10 antibody 16936-1-APDownload protocol
IHC protocol for RNF10 antibody 16936-1-APDownload protocol
IF protocol for RNF10 antibody 16936-1-APDownload protocol
IP protocol for RNF10 antibody 16936-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Elife

Ring finger protein 10 is a novel synaptonuclear messenger encoding activation of NMDA receptors in hippocampus.

Authors - Margarita C Dinamarca
  • KD Validated
View Article
humanWB

Cell Rep

The E3 ubiquitin ligase RNF10 modifies 40S ribosomal subunits of ribosomes compromised in translation.

Authors - Aitor Garzia
  • KO Validated
View Article
mouse,humanWB

Nat Cell Biol

Gut microbial metabolite facilitates colorectal cancer development via ferroptosis inhibition

Authors - Weiwei Cui
View Article
mouse

J Clin Invest

RNF4 sustains Myc-driven tumorigenesis by facilitating DNA replication

Authors - Joonyoung Her
View Article
ratWB,IHC

Mol Neurobiol

The Synaptonuclear Messenger RNF10 Acts as an Architect of Neuronal Morphology.

Authors - Nicolò Carrano
View Article