验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, Jurkat cells, human brain tissue |
| Positive IP detected in | PC-3 cells |
| Positive IHC detected in | mouse brain tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16936-1-AP targets RNF10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | RNF10 fusion protein Ag10433 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ring finger protein 10 |
| 别名 | RING finger protein 10, RIE2, KIAA0262, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF10 |
| 计算分子量 | 811 aa, 90 kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC016622 |
| 基因名称 | RNF10 |
| Gene ID (NCBI) | 9921 |
| RRID | AB_2181846 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N5U6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RING finger (C3HC4-type zinc finger) is a variant zinc finger motif present in a new family of proteins including transcription regulators. RNF10 (RING finger protein 10), a 90-kDa protein, contains a ring finger motif which is known to be involved in protein-protein interactions. RNF10 regulates myelin-associated glycoprotein (MAG) expression and is required for myelin formation, therefore, participates in the peripheral nerve development and Schawnn cell differentiation. RNF10 is also involved in activation of p21WAF1 promoter via its activity as transcriptional factor.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF10 antibody 16936-1-AP | Download protocol |
| IHC protocol for RNF10 antibody 16936-1-AP | Download protocol |
| IF protocol for RNF10 antibody 16936-1-AP | Download protocol |
| IP protocol for RNF10 antibody 16936-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Ring finger protein 10 is a novel synaptonuclear messenger encoding activation of NMDA receptors in hippocampus.
| ||
Cell Rep The E3 ubiquitin ligase RNF10 modifies 40S ribosomal subunits of ribosomes compromised in translation.
| ||
Nat Cell Biol Gut microbial metabolite facilitates colorectal cancer development via ferroptosis inhibition | ||
Nat Commun E3 ubiquitin ligase RNF10 promotes dissociation of stalled ribosomes and responds to ribosomal subunit imbalance | ||
Mol Neurobiol The Synaptonuclear Messenger RNF10 Acts as an Architect of Neuronal Morphology. |
