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验证数据展示

  • WB analysis using 14338-1-AP

    WB analysis using 14338-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14338-1-AP (RNF114 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 14338-1-AP

    IHC staining of human ovary cancer using 14338-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 14338-1-AP (RNF114 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of BGC-823 using 14338-1-AP

    IF Staining of BGC-823 using 14338-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed BGC-823 cells using RNF114 antibody (14338-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

RNF114 Polyclonal antibody

RNF114 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14338-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

ZNF313, ZNF228, RING-type E3 ubiquitin transferase RNF114, PSORS12, EC:2.3.2.27

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, MCF-7 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBGC-823 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14338-1-AP targets RNF114 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 RNF114 fusion protein Ag5649 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 114
别名 ZNF313, ZNF228, RING-type E3 ubiquitin transferase RNF114, PSORS12, EC:2.3.2.27
计算分子量 26 kDa
观测分子量26 kDa
GenBank蛋白编号BC066919
基因名称 RNF114
Gene ID (NCBI) 55905
RRIDAB_3085435
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y508
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF114, also known as ZNF228 or ZNF313, is a member of the zinc-binding protein family. RNF114 contains two C2H2 and Ring finger zinc-binding domains and a ubiquitin-interacting motif (UIM). RNF114 functions as an E3 ligase in the ubiquitin-proteasome system and plays an important role in basic cellular processes in different types of cells. RNF114 is expressed widely in many normal tissues, such as the heart, liver, and kidney. RNF114 has 2 isoforms with the molecular mass of 21 and 26 kDa. RNF114 is a novel determinant of psoriasis susceptibility, and its overexpression is a crucial indicator of epithelial inflammation (PMID: 34104941, 35069903, 37523804).

实验方案

Product Specific Protocols
WB protocol for RNF114 antibody 14338-1-APDownload protocol
IHC protocol for RNF114 antibody 14338-1-APDownload protocol
IF protocol for RNF114 antibody 14338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols