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验证数据展示

  • WB analysis using 25260-1-AP

    WB analysis using 25260-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25260-1-AP (RNF123 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 25260-1-AP

    IP experiment of HeLa using 25260-1-AP

    IP result of anti-RNF123 (IP:25260-1-AP, 4ug; Detection:25260-1-AP 1:600) with HeLa cells lysate 1200 ug.

RNF123 Polyclonal antibody

RNF123 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25260-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

ring finger protein 123, KPC1, FP1477

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, HuH-7 cells
Positive IP detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RNF123 Ready-To-Use IHC Kit. RNF123 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25260-1-AP targets RNF123 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19653

Product name: Recombinant human RNF123 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 12-358 aa of BC130632

Sequence: RKSYRLTSDAEKSRVTGIVQEKLLNDYLNRIFSSSEHAPPAATSRKPLNFQNLPEHLDQLLQVDNEEEESQGQVEGRLGPSTVVLDHTGGFEGLLLVDDDLLGVIGHSNFGTIRSTTCVYKGKWLYEVLISSQGLMQIGWCTISCRFNQEEGVGDTHNSYAYDGNRVRKWNVTTTNYGKAWAAGDIVSCLIDLDDGTLSFCLNGVSLGTAFENLSRGLGMAYFPAISLSFKESVAFNFGSRPLRYPVAGYRPLQDPPSADLVRAQRLLGCFRAVLSVELDPVEGRLLDKESSKWRLRGQPTVLLTLAHIFHHFAPLLRKVYLVEAVLMSFLLGIVEKGTPTQAQSVV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 123
别名 ring finger protein 123, KPC1, FP1477
计算分子量 1314 aa, 149 kDa
观测分子量149 kDa
GenBank蛋白编号BC130632
基因名称 RNF123
Gene ID (NCBI) 63891
RRIDAB_3669467
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5XPI4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RING finger protein 123 (RNF123), also known as KPC1, is an E3 ubiquitin ligase that belongs to the RING-finger family of ligases that contain a 40-60-amino acid long RING domain which is essential for causing substrate ubiquitination (PMID: 29676528). RNF123 promoted TLR3/IRF7-induced type I IFN signaling and inhibited viral replication by degrading SOCS1 (PMID: 37014223).

实验方案

Product Specific Protocols
IP protocol for RNF123 antibody 25260-1-APDownload protocol
WB protocol for RNF123 antibody 25260-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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