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RNF133 Recombinant antibody

RNF133 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240397C12

Cat no : 83462-1-RR

Print datasheet

Synonyms

RING-type E3 ubiquitin transferase RNF133, RING finger protein 133, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF133, 240397C12

验证数据展示

  • WB analysis using 83462-1-RR

    WB analysis using 83462-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83462-1-RR (RNF133 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83462-1-RR

    Affinity and Kinetic Characterization of 83462-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83462-1-RR against Human RNF133 were performed. The affinity constant is 0.714 nM.

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83462-1-RR is RNF133 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen RNF133 fusion protein Ag14537 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ring finger protein 133
Synonyms RING-type E3 ubiquitin transferase RNF133, RING finger protein 133, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF133, 240397C12
Calculated Molecular Weight 376 aa, 42 kDa
Observed Molecular Weight47 kDa
GenBank Accession NumberBC022038
Gene Symbol RNF133
Gene ID (NCBI) 168433
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8WVZ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF133 (ring finger protein 133) contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions.

实验方案

Product Specific Protocols
WB protocol for RNF133 antibody 83462-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols