• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RNF133 Recombinant antibody

RNF133 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240397C12

Cat no : 83462-1-RR

Print datasheet

Synonyms

RING-type E3 ubiquitin transferase RNF133, RING finger protein 133, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF133, 240397C12

验证数据展示

  • WB analysis using 83462-1-RR

    WB analysis using 83462-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83462-1-RR (RNF133 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83462-1-RR

    Affinity and Kinetic Characterization of 83462-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83462-1-RR against Human RNF133 were performed. The affinity constant is 0.714 nM.

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83462-1-RR targets RNF133 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen RNF133 fusion protein Ag14537 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ring finger protein 133
Synonyms RING-type E3 ubiquitin transferase RNF133, RING finger protein 133, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF133, 240397C12
Calculated Molecular Weight 376 aa, 42 kDa
Observed Molecular Weight47 kDa
GenBank Accession NumberBC022038
Gene Symbol RNF133
Gene ID (NCBI) 168433
RRIDAB_3671093
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8WVZ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF133 (ring finger protein 133) contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions.

实验方案

Product Specific Protocols
WB protocol for RNF133 antibody 83462-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols