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验证数据展示

  • IF Staining of PC-12 using 84984-1-RR

    IF Staining of PC-12 using 84984-1-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using RNF135 antibody (84984-1-RR, Clone: 241606D8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84984-1-RR

    Affinity and Kinetic Characterization of 84984-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84984-1-RR against Human RNF135 were performed. The affinity constant is 49.1 nM.

RNF135 Recombinant antibody

RNF135 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 84984-1-RR

Print datasheet

CloneNo. 241606D8

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, ELISA

Synonyms

RIG I E3 ubiquitin ligase, REUL, L13, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF135

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inPC-12 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84984-1-RR targets RNF135 in IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human, rat
Immunogen RNF135 fusion protein Ag18821 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ring finger protein 135
Synonyms RIG I E3 ubiquitin ligase, REUL, L13, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF135
Calculated Molecular Weight 432 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC005084
Gene Symbol RNF135
Gene ID (NCBI) 84282
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IUD6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ring finger protein 135 (RNF135), located on chromosome 17q11.2, is a RING finger domain-containing E3 ubiquitin ligase that was identified as a bio-marker and therapy target of glioblastoma (PMID: 26856755). RNF135 facilitates RIG-I C-terminal ubiquitination to up-regulate RIG-I-mediated IFN signaling and suppress viral replication.

实验方案

Product Specific Protocols
IF protocol for RNF135 antibody 84984-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols