RNF139 Polyclonal antibody

RNF139 Polyclonal Antibody for WB, ELISA
Cat No. 28261-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

RING-type E3 ubiquitin transferase RNF139, RING finger protein 139, RCA1, HRCA1, EC:2.3.2.27

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HT-29 cells, HepG2 cells, THP-1 cells, U-87 MG cells, U2OS cells
Planning an IHC experiment? We recommend our IHCeasy RNF139 Ready-To-Use IHC Kit. RNF139 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28261-1-AP targets RNF139 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 RNF139 fusion protein Ag27147 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 139
别名 RING-type E3 ubiquitin transferase RNF139, RING finger protein 139, RCA1, HRCA1, EC:2.3.2.27
计算分子量 664 aa, 76 kDa
观测分子量70 kDa
GenBank蛋白编号BC064636
基因名称 RNF139
Gene ID (NCBI) 11236
RRIDAB_3086042
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WU17
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The RNF139 gene (ring-finger protein 139), also known as TRC8 (translocation in renal carcinoma from chromosome 8), was first identified from a family with 3;8 chromosomal translocation and hereditary renal cell carcinoma and thyroid cancer (PMID:22689053). RNF139 encodes an endoplasmic reticulum (ER) protein with 10 transmembrane segments that contain a sterol-sensing domain and a RING finger motif encoding an E3 ubiquitin ligase (PMID: 19706601). Northern blot and dot blot show that RNF139 is highly expressed in the testis, placenta, and adrenal gland and is expressed at lower levels in the heart, brain, liver, skeletal muscle, and pancreas (PMID: 9689122). It is reported that TRC8 suppresses tumorigenesis by targeting heme oxygenase-1 (HO-1), an antioxidant enzyme highly expressed in various cancers, for ubiquitination and degradation (PMID:22689053). TRC8 is also involved in cholesterol and fatty acid biosynthesis that are transcriptionally regulated by the sterol response element binding proteins (SREBPs) (PMID:17016439). Acetylation, phosphorylation, and autoubiquitination are common post-translational modifications of FNF139 protein.

实验方案

Product Specific Protocols
WB protocol for RNF139 antibody 28261-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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