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验证数据展示

  • WB analysis using 28261-1-AP

    WB analysis using 28261-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28261-1-AP (RNF139 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

RNF139 Polyclonal antibody

RNF139 Polyclonal Antibody for WB, ELISA
Cat No. 28261-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

RING-type E3 ubiquitin transferase RNF139, RING finger protein 139, RCA1, HRCA1, EC:2.3.2.27

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated

Size: 
50 μL
100 μL
150 μL

¥2200/ 100 μL

浓度: 700 ug/ml

库存：现货

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经过测试的应用

Positive WB detected inHEK-293 cells, HT-29 cells, HepG2 cells, THP-1 cells, U-87 MG cells, U2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28261-1-AP targets RNF139 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen RNF139 fusion protein Ag27147 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 139
Synonyms RING-type E3 ubiquitin transferase RNF139, RING finger protein 139, RCA1, HRCA1, EC:2.3.2.27
Calculated Molecular Weight 664 aa, 76 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC064636
Gene Symbol RNF139
Gene ID (NCBI) 11236
RRIDAB_3086042
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WU17
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The RNF139 gene (ring-finger protein 139), also known as TRC8 (translocation in renal carcinoma from chromosome 8), was first identified from a family with 3;8 chromosomal translocation and hereditary renal cell carcinoma and thyroid cancer (PMID:22689053). RNF139 encodes an endoplasmic reticulum (ER) protein with 10 transmembrane segments that contain a sterol-sensing domain and a RING finger motif encoding an E3 ubiquitin ligase (PMID: 19706601). Northern blot and dot blot show that RNF139 is highly expressed in the testis, placenta, and adrenal gland and is expressed at lower levels in the heart, brain, liver, skeletal muscle, and pancreas (PMID: 9689122). It is reported that TRC8 suppresses tumorigenesis by targeting heme oxygenase-1 (HO-1), an antioxidant enzyme highly expressed in various cancers, for ubiquitination and degradation (PMID:22689053). TRC8 is also involved in cholesterol and fatty acid biosynthesis that are transcriptionally regulated by the sterol response element binding proteins (SREBPs) (PMID:17016439). Acetylation, phosphorylation, and autoubiquitination are common post-translational modifications of FNF139 protein.

实验方案

Product Specific Protocols
WB protocol for RNF139 antibody 28261-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols