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验证数据展示

  • WB analysis of HEK-293T using 26368-1-AP

    WB analysis of HEK-293T using 26368-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 26368-1-AP (RNF14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of COLO 320 using 26368-1-AP

    IP experiment of COLO 320 using 26368-1-AP

    IP result of anti-RNF14 (IP:26368-1-AP, 4ug; Detection:26368-1-AP 1:600) with COLO 320 cells lysate 4000ug.

  • IHC staining of human colon cancer using 26368-1-AP

    IHC staining of human colon cancer using 26368-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26368-1-AP (RNF14 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HEK-293 using 26368-1-AP

    IF Staining of HEK-293 using 26368-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RNF14 antibody (26368-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RNF14 Polyclonal antibody

RNF14 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 26368-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

HRIHFB2038, HFB30, EC:2.3.2.31, E3 ubiquitin-protein ligase RNF14, ARA54

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, COLO 320 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26368-1-AP targets RNF14 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 RNF14 fusion protein Ag23734 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 14
别名 HRIHFB2038, HFB30, EC:2.3.2.31, E3 ubiquitin-protein ligase RNF14, ARA54
观测分子量 47-54 kDa
GenBank蛋白编号BC126185
基因名称 RNF14
Gene ID (NCBI) 9604
RRIDAB_2880494
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UBS8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RNF14 antibody 26368-1-APDownload protocol
IHC protocol for RNF14 antibody 26368-1-APDownload protocol
IF protocol for RNF14 antibody 26368-1-APDownload protocol
IP protocol for RNF14 antibody 26368-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols