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RNF144A Polyclonal antibody

RNF144A Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, hamster

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 26144-1-AP

Print datasheet

Synonyms

UbcM4-interacting protein 4, UBCE7IP4, RNF144, KIAA0161, EC:2.3.2.31

验证数据展示

  • WB analysis of MDA-MB-231 using 26144-1-AP

    WB analysis of MDA-MB-231 using 26144-1-AP

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 26144-1-AP (RNF144A antibody) at dilution of 1:1500 and incubated at room temperature for 1.5 hours.

  • IP experiment of CHO using 26144-1-AP

    IP experiment of CHO using 26144-1-AP

    IP result of anti-RNF144A (IP:26144-1-AP, 4ug; Detection:26144-1-AP 1:300) with CHO cells lysate 3100ug.

经过测试的应用

Positive WB detected inMDA-MB-231 cells
Positive IP detected inCHO cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

26144-1-AP targets RNF144A in WB, IP, ELISA applications and shows reactivity with human, hamster samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, hamster
Cited Reactivityhuman
Immunogen RNF144A fusion protein Ag23508 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 144A
Synonyms UbcM4-interacting protein 4, UBCE7IP4, RNF144, KIAA0161, EC:2.3.2.31
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC050373
Gene Symbol RNF144A
Gene ID (NCBI) 9781
RRIDAB_2880402
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50876
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF144A (Ring Finger protein 144A) as the first E3 ubiquitin ligase for cytosolic DNA-PKcs, is induced in a p53-dependent manner during DNA damage and targets cytosolic DNA-PKcs for ubiquitination and degradation. RNF144A is an RBR-containing protein, and is unstable and degraded through the proteasome-dependent pathway (PMID: 24979766). RNF144A contains an RBR domain in the N-terminus and a potential single-transmembrane (TM) domain in the C-terminus, and is distributed in both plasma membrane and the membranes of intracellular vesicles including endosomes, lysosomes, endoplasmic reticulum (ER), perinuclear vesicles, and others, which might be caused by the presence of the TM domain (PMID: 37955227).

实验方案

Product Specific Protocols
WB protocol for RNF144A antibody 26144-1-APDownload protocol
IP protocol for RNF144A antibody 26144-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO Rep

RNF144A promotes antiviral responses by modulating STING ubiquitination

Authors - Bo Yang
View Article