验证数据展示
经过测试的应用
| Positive WB detected in | MDA-MB-231 cells |
| Positive IP detected in | CHO cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
26144-1-AP targets RNF144A in WB, IP, ELISA applications and shows reactivity with human, hamster samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, hamster |
| 文献引用反应性 | human |
| 免疫原 | RNF144A fusion protein Ag23508 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ring finger protein 144A |
| 别名 | UbcM4-interacting protein 4, UBCE7IP4, RNF144, KIAA0161, EC:2.3.2.31 |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC050373 |
| 基因名称 | RNF144A |
| Gene ID (NCBI) | 9781 |
| RRID | AB_2880402 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P50876 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNF144A (Ring Finger protein 144A) as the first E3 ubiquitin ligase for cytosolic DNA-PKcs, is induced in a p53-dependent manner during DNA damage and targets cytosolic DNA-PKcs for ubiquitination and degradation. RNF144A is an RBR-containing protein, and is unstable and degraded through the proteasome-dependent pathway (PMID: 24979766). RNF144A contains an RBR domain in the N-terminus and a potential single-transmembrane (TM) domain in the C-terminus, and is distributed in both plasma membrane and the membranes of intracellular vesicles including endosomes, lysosomes, endoplasmic reticulum (ER), perinuclear vesicles, and others, which might be caused by the presence of the TM domain (PMID: 37955227).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF144A antibody 26144-1-AP | Download protocol |
| IP protocol for RNF144A antibody 26144-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy RNF144A inhibits autophagy by targeting BECN1 for degradation during L. monocytogenes infection |
