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RNF144B/IBRDC2 Polyclonal antibody

RNF144B/IBRDC2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 26306-1-AP

Print datasheet

Synonyms

IBRDC2, KIAA0161, p53RFP, ring finger protein 144B, RNF144B

验证数据展示

  • WB analysis of HeLa using 26306-1-AP

    WB analysis of HeLa using 26306-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26306-1-AP (RNF144B/IBRDC2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 26306-1-AP

    IP experiment of HeLa using 26306-1-AP

    IP result of anti-RNF144B/IBRDC2 (IP:26306-1-AP, 4ug; Detection:26306-1-AP 1:300) with HeLa cells lysate 2400ug.

  • IHC staining of human kidney using 26306-1-AP

    IHC staining of human kidney using 26306-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 26306-1-AP

    IHC staining of human kidney using 26306-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26306-1-AP is RNF144B/IBRDC2 Fusion Protein expressed in E. coli.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IP, CoIP
Tested Reactivity human
Cited Reactivityhuman
Immunogen RNF144B/IBRDC2 fusion protein Ag23679 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 144B
Synonyms IBRDC2, KIAA0161, p53RFP, ring finger protein 144B, RNF144B
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC063311
Gene Symbol RNF144B
Gene ID (NCBI) 255488
RRIDAB_2880473
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z419
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RNF144B/IBRDC2 antibody 26306-1-APDownload protocol
IHC protocol for RNF144B/IBRDC2 antibody 26306-1-APDownload protocol
IP protocol for RNF144B/IBRDC2 antibody 26306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP,CoIP

EMBO Rep

RNF144B negatively regulates antiviral immunity by targeting MDA5 for autophagic degradation

Authors - Guoxiu Li
View Article