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验证数据展示

  • WB analysis of mouse lung using 21438-1-AP

    WB analysis of mouse lung using 21438-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21438-1-AP (RNF150 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 21438-1-AP

    IHC staining of human colon using 21438-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21438-1-AP (RNF150 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 21438-1-AP

    IHC staining of human colon using 21438-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21438-1-AP (RNF150 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RNF150 Polyclonal antibody

RNF150 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21438-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

别名

KIAA1214, ring finger protein 150, RNF150

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse lung tissue, human heart tissue, mouse brain tissue, mouse eye tissue, Y79 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21438-1-AP targets RNF150 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 RNF150 fusion protein Ag16064 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 150
别名 KIAA1214, ring finger protein 150, RNF150
计算分子量 438 aa, 48 kDa
观测分子量 43-44 kDa
GenBank蛋白编号BC101992
基因名称 RNF150
Gene ID (NCBI) 57484
RRIDAB_10733469
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9ULK6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The RING finger (RNF) family, containing the N-terminal RING domain, is the largest E3 ubiquitin ligase family with 340 validated human members (PMID: 25609945). RNF150 proteins belong to the Goliath family and are involved in apoptosis and embryonic development (PMID: 19690564). RNF150 might be involved in the progression of GC partly via heparin binding which correlates well with angiogenesis and hematogenous metastasis (PMID: 37528105). There are four isoforms of RNF150, 48kDa, 44kDa, 43kDa, 32 kDa respectively.

实验方案

Product Specific Protocols
WB protocol for RNF150 antibody 21438-1-APDownload protocol
IHC protocol for RNF150 antibody 21438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

Cell Biol Int

RNF150 suppresses papillary thyroid carcinoma with ASK1 ubiquitination presenting a direct target via inactivating p38 signaling axis

Authors - Wei Deng
View Article