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RNF151 Polyclonal antibody

RNF151 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24708-1-AP

Print datasheet

Synonyms

ring finger protein 151, RNF151

验证数据展示

  • IHC staining of mouse testis using 24708-1-AP

    IHC staining of mouse testis using 24708-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24708-1-AP (RNF151 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of rat testis using 24708-1-AP

    WB analysis of rat testis using 24708-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 24708-1-AP (RNF151 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inrat testis tissue, human testis tissue, mouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24708-1-AP is RNF151 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RNF151 fusion protein Ag16606 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 151
Synonyms ring finger protein 151, RNF151
Calculated Molecular Weight 245 aa, 27 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC113014
Gene Symbol RNF151
Gene ID (NCBI) 146310
RRIDAB_2879683
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2KHN1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF151 also termed as ring finger protein 151 is a 245 amino acid protein, which contains one Ring-type zinc finger and one TRAF-type zinc finger. RNF151, with, a putative nuclear localization signal (NLS)was exclusively expressed in the mouse testis and developmentally regulated during spermatogenesis. While RNF151 mRNA was present in round spermatids, its protein was expressed in elongating spermatids of the stage VIII-IX seminiferous tubules.

实验方案

Product Specific Protocols
WB protocol for RNF151 antibody 24708-1-APDownload protocol
IHC protocol for RNF151 antibody 24708-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols