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验证数据展示

  • IHC staining of mouse testis using 24708-1-AP

    IHC staining of mouse testis using 24708-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24708-1-AP (RNF151 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of rat testis using 24708-1-AP

    WB analysis of rat testis using 24708-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 24708-1-AP (RNF151 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

RNF151 Polyclonal antibody

RNF151 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24708-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ring finger protein 151, RNF151

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat testis tissue, human testis tissue, mouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24708-1-AP targets RNF151 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RNF151 fusion protein Ag16606 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 151
别名 ring finger protein 151, RNF151
计算分子量 245 aa, 27 kDa
观测分子量 26 kDa
GenBank蛋白编号BC113014
基因名称 RNF151
Gene ID (NCBI) 146310
RRIDAB_2879683
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ2KHN1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF151 also termed as ring finger protein 151 is a 245 amino acid protein, which contains one Ring-type zinc finger and one TRAF-type zinc finger. RNF151, with, a putative nuclear localization signal (NLS)was exclusively expressed in the mouse testis and developmentally regulated during spermatogenesis. While RNF151 mRNA was present in round spermatids, its protein was expressed in elongating spermatids of the stage VIII-IX seminiferous tubules.

实验方案

Product Specific Protocols
WB protocol for RNF151 antibody 24708-1-APDownload protocol
IHC protocol for RNF151 antibody 24708-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols