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RNF160 Polyclonal antibody

RNF160 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28452-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

C21orf10, C21orf98, E3 ubiquitin-protein ligase listerin, EC:2.3.2.27, HSPC087

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inBxPC-3 cells, HepG2 cells, HeLa cells, mouse pancreas tissue, rat pancreas tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 7 publications below

产品信息

28452-1-AP targets RNF160 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 RNF160 fusion protein Ag29467 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 160
别名 C21orf10, C21orf98, E3 ubiquitin-protein ligase listerin, EC:2.3.2.27, HSPC087
观测分子量 180-200 kDa
GenBank蛋白编号BC140790
基因名称 RNF160
Gene ID (NCBI) 26046
RRIDAB_2881144
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94822
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The E3 ubiquitin ligase, Ltn1 (also named RNF160), is a key regulator of RQC that targets substrates for ubiquitination (PMID: 35981909).

实验方案

Product Specific Protocols
WB protocol for RNF160 antibody 28452-1-APDownload protocol
IHC protocol for RNF160 antibody 28452-1-APDownload protocol
IF protocol for RNF160 antibody 28452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Nat Commun

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis

Authors - Ji Geng
humanWB

Cell Rep

NEMF-mediated Listerin-independent mitochondrial translational surveillance by E3 ligase Pirh2 and mitochondrial protease ClpXP

Authors - Liang Lv
humanWB

Acta Neuropathol Commun

Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease

Authors - Suman Rimal
human

Cell Rep

The E3 ubiquitin ligase FBXL6 controls the quality of newly synthesized mitochondrial ribosomal proteins

Authors - Julie Lavie
humanWB

bioRxiv

Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats

Authors - Yi-Ju Tseng
humanWB

bioRxiv

Nascent Chain Ubiquitination is Uncoupled from Degradation to Enable Protein Maturation

Authors - Xia Li
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