验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, HeLa cells, HepG2 cells, mouse pancreas tissue, rat pancreas tissue |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
产品信息
28452-1-AP targets RNF160 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, rat, mouse |
| Cited Reactivity | human |
| Immunogen | RNF160 fusion protein Ag29467 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ring finger protein 160 |
| Synonyms | C21orf10, C21orf98, E3 ubiquitin-protein ligase listerin, FLJ11053, KIAA0714, LTN1, ring finger protein 160, RNF160, Zinc finger protein 294, ZNF294 |
| Observed Molecular Weight | 180-200 kDa |
| GenBank Accession Number | BC140790 |
| Gene Symbol | RNF160 |
| Gene ID (NCBI) | 26046 |
| RRID | AB_2881144 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94822 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The E3 ubiquitin ligase, Ltn1 (also named RNF160), is a key regulator of RQC that targets substrates for ubiquitination (PMID: 35981909).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF160 antibody 28452-1-AP | Download protocol |
| IHC protocol for RNF160 antibody 28452-1-AP | Download protocol |
| IF protocol for RNF160 antibody 28452-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis | ||
Cell Rep NEMF-mediated Listerin-independent mitochondrial translational surveillance by E3 ligase Pirh2 and mitochondrial protease ClpXP | ||
Acta Neuropathol Commun Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease | ||
Cell Rep The E3 ubiquitin ligase FBXL6 controls the quality of newly synthesized mitochondrial ribosomal proteins | ||
bioRxiv Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats | ||
bioRxiv Nascent Chain Ubiquitination is Uncoupled from Degradation to Enable Protein Maturation |