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验证数据展示

  • WB analysis using 68610-1-Ig

    WB analysis using 68610-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68610-1-Ig (RNF160 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

RNF160 Monoclonal antibody

RNF160 Monoclonal Antibody for WB, ELISA
Cat No. 68610-1-Ig

产品说明书

CloneNo. 1D2A2

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat, mouse

应用

WB, ELISA

别名

C21orf10, C21orf98, FLJ11053, KIAA0714, ring finger protein 160, RNF160, Zinc finger protein 294, ZNF294

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, U2OS cells, HSC-T6 cells, NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68610-1-Ig targets RNF160 in WB, ELISA applications and shows reactivity with Human, rat, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, rat, mouse
免疫原 RNF160 fusion protein Ag29471 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ring finger protein 160
别名 C21orf10, C21orf98, FLJ11053, KIAA0714, ring finger protein 160, RNF160, Zinc finger protein 294, ZNF294
观测分子量180-200 kDa
GenBank蛋白编号BC140790
基因名称 RNF160
Gene ID (NCBI) 26046
RRIDAB_3085305
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO94822
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The E3 ubiquitin ligase, Ltn1 (also named RNF160), is a key regulator of RQC that targets substrates for ubiquitination (PMID: 35981909).

实验方案

Product Specific Protocols
WB protocol for RNF160 antibody 68610-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols