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RNF170 Polyclonal antibody

RNF170 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21024-1-AP

Print datasheet

Synonyms

ring finger protein 170, RNF170

验证数据展示

  • WB analysis of human placenta using 21024-1-AP

    WB analysis of human placenta using 21024-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 21024-1-AP (RNF170 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 21024-1-AP

    IHC staining of human breast cancer using 21024-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21024-1-AP (RNF170 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 21024-1-AP

    IHC staining of human breast cancer using 21024-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21024-1-AP (RNF170 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21024-1-AP targets RNF170 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen RNF170 fusion protein Ag15288 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 170
Synonyms ring finger protein 170, RNF170
Calculated Molecular Weight 258 aa, 30 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC032393
Gene Symbol RNF170
Gene ID (NCBI) 81790
RRIDAB_10733119
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96K19
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF170 is an E3 ubiquitin ligase that resides in the endoplasmic reticulum (ER) membrane and mediates ubiquitination and processing of inositol 1,4,5-trisphosphate (IP3) receptors by the ER-associated protein degradation (ERAD) pathway. It has 6 isoforms produced by Alternative splicing with the molecular weight of 30 kDa, 27 kDa, 22 kDa, 20 kDa, 19 kDa, and 13 kDa.

实验方案

Product Specific Protocols
WB protocol for RNF170 antibody 21024-1-APDownload protocol
IHC protocol for RNF170 antibody 21024-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols