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验证数据展示

  • WB analysis of human placenta using 21024-1-AP

    WB analysis of human placenta using 21024-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 21024-1-AP (RNF170 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 21024-1-AP

    IHC staining of human breast cancer using 21024-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21024-1-AP (RNF170 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 21024-1-AP

    IHC staining of human breast cancer using 21024-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21024-1-AP (RNF170 antibody) at dilution of 1:50 (under 40x lens).

RNF170 Polyclonal antibody

RNF170 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21024-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

ring finger protein 170, RNF170

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21024-1-AP targets RNF170 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 RNF170 fusion protein Ag15288 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 170
别名 ring finger protein 170, RNF170
计算分子量 258 aa, 30 kDa
观测分子量 27 kDa
GenBank蛋白编号BC032393
基因名称 RNF170
Gene ID (NCBI) 81790
RRIDAB_10733119
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96K19
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF170 is an E3 ubiquitin ligase that resides in the endoplasmic reticulum (ER) membrane and mediates ubiquitination and processing of inositol 1,4,5-trisphosphate (IP3) receptors by the ER-associated protein degradation (ERAD) pathway. It has 6 isoforms produced by Alternative splicing with the molecular weight of 30 kDa, 27 kDa, 22 kDa, 20 kDa, 19 kDa, and 13 kDa.

实验方案

Product Specific Protocols
WB protocol for RNF170 antibody 21024-1-APDownload protocol
IHC protocol for RNF170 antibody 21024-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols