验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, L02 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human kidney tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
20408-1-AP targets RNF181 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 免疫原 | RNF181 fusion protein Ag14233 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ring finger protein 181 |
| 别名 | RN181, RING finger protein 181, HSPC238, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF181 |
| 计算分子量 | 153 aa, 18 kDa |
| 观测分子量 | 18 kDa |
| GenBank蛋白编号 | BC002803 |
| 基因名称 | RNF181 |
| Gene ID (NCBI) | 51255 |
| RRID | AB_10696314 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9P0P0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNF181, also named as HSPC238, is a 153 amino acid protein, which belongs to the RNF181 family. RNF181 is widely expressed, with highest levels in liver and heart and lowest levels in brain and skeletal muscle. RNF181 as a E3 ubiquitin-protein ligase, accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Western blot analysis detected RNF181 protein in human platelets as a 36-kD dimer and an 18-kD monomer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF181 antibody 20408-1-AP | Download protocol |
| IHC protocol for RNF181 antibody 20408-1-AP | Download protocol |
| IF protocol for RNF181 antibody 20408-1-AP | Download protocol |
| IP protocol for RNF181 antibody 20408-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
