验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21178-1-AP targets RNF212 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | RNF212 fusion protein Ag15457 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ring finger protein 212 |
| 别名 | ring finger protein 212, RNF212, ZHP3 |
| 计算分子量 | 297 aa, 33 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC050356 |
| 基因名称 | RNF212 |
| Gene ID (NCBI) | 285498 |
| RRID | AB_10863660 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q495C1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNF212(RING finger protein 212)acts as a regulator of crossing-over during meiosis: required to couple chromosome synapsis to the formation of crossover-specific recombination complexes. It may be a SUMO E3 ligase that mediates the sumoylation of target proteins MSH4 and MSH5, leading to enhanced binding to recombination sites.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF212 antibody 21178-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |