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RNF213 Polyclonal antibody

RNF213 Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21028-1-AP

Print datasheet

Synonyms

ALO17, C17orf27, KIAA1554, KIAA1618, Protein ALO17, ring finger protein 213, RNF213

验证数据展示

  • IHC staining of human lymphoma using 21028-1-AP

    IHC staining of human lymphoma using 21028-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma slide using 21028-1-AP (RNF213 Antibody) at dilution of 1:50.

  • IHC staining of human lymphoma using 21028-1-AP

    IHC staining of human lymphoma using 21028-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma slide using 21028-1-AP (RNF213 Antibody) at dilution of 1:50.

  • IP experiment of HUVEC using 21028-1-AP

    IP experiment of HUVEC using 21028-1-AP

    IP result of anti-RNF213 (IP:21028-1-AP, 4ug; Detection:21028-1-AP 1:300) with HUVEC cells lysate 910 ug.

经过测试的应用

Positive IP detected inHUVEC cells
Positive IHC detected inhuman lymphoma tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

21028-1-AP targets RNF213 in IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IP, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen RNF213 fusion protein Ag15335 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 213
Synonyms ALO17, C17orf27, KIAA1554, KIAA1618, Protein ALO17, ring finger protein 213, RNF213
Calculated Molecular Weight 3280 aa, 374 kDa
GenBank Accession NumberBC032220
Gene Symbol RNF213
Gene ID (NCBI) 57674
RRIDAB_2878793
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ63HN8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF213 is a typical E3 ubiquitin ligase that can catalyze ubiquitination of both proteins and lipids, and which is involved in various processes, such as lipid metabolism, angiogenesis and cell-autonomous immunity (PMID: 21799892, 26126547, 26278786, 26766444, 30705059).RNF213 contains a C3HC4-type RING finger domain, which is a specialized type of Zn-finger that binds two atoms of zinc and is thought to be involved in mediating protein-protein interactions.

实验方案

Product Specific Protocols
IHC protocol for RNF213 antibody 21028-1-APDownload protocol
IP protocol for RNF213 antibody 21028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Thorac Cancer

RNF213 gene mutation in circulating tumor DNA detected by targeted next-generation sequencing in the assisted discrimination of early-stage lung cancer from pulmonary nodules.

Authors - Ning Jiang
View Article