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RNF216 Polyclonal antibody

RNF216 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10185-1-AP

Print datasheet

Synonyms

TRIAD3, Triad domain-containing protein 3, RING-type E3 ubiquitin transferase RNF216, RING finger protein 216, EC:2.3.2.27

验证数据展示

  • WB analysis of U2OS using 10185-1-AP

    WB analysis of U2OS using 10185-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 10185-1-AP (RNF216 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of SW480 using 10185-1-AP

    WB analysis of SW480 using 10185-1-AP

    SW480 cells were subjected to SDS PAGE followed by western blot with 10185-1-AP (RNF216 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10185-1-AP

    WB analysis using 10185-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10185-1-AP (RNF216 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of SW480 using 10185-1-AP

    IP experiment of SW480 using 10185-1-AP

    IP result of anti-RNF216 (IP:10185-1-AP, 4ug; Detection:10185-1-AP 1:1000) with SW480 cells lysate 1280 ug.

  • IF Staining of A431 using 10185-1-AP

    IF Staining of A431 using 10185-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RNF216 antibody (10185-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inmouse heart tissue, U2OS cells, SW480 cells, mouse kidney tissue, rat heart tissue
Positive IP detected inSW480 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10185-1-AP targets RNF216 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RNF216 fusion protein Ag0238 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 216
Synonyms TRIAD3, Triad domain-containing protein 3, RING-type E3 ubiquitin transferase RNF216, RING finger protein 216, EC:2.3.2.27
Calculated Molecular Weight 48 kDa
Observed Molecular Weight57 kDa, 90-99 kDa
GenBank Accession NumberBC000787
Gene Symbol RNF216
Gene ID (NCBI) 54476
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWF9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF216/TRIAD3 is an RBR (RING-between-RING) E3 ligase that encodes for multiple isoforms that include TRIAD3A, TRIAD3B, TRIAD3C and TRIAD3D/E. RNF216 is a broadly expressed RBR E3 ligase, and loss-of-function mutations in RNF216 are linked to the neurode-generative conditions Gordon-Holmes syndrome (GHS) and Huntington-like disorders (PMID: 34998453, 35620441). RNF216 has three different molecular weights, 99 kDa, 106 kDa and 57 kDa, respectively.

实验方案

Product Specific Protocols
WB protocol for RNF216 antibody 10185-1-APDownload protocol
IF protocol for RNF216 antibody 10185-1-APDownload protocol
IP protocol for RNF216 antibody 10185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Mol Med

Xanthatin synergizes with cisplatin to suppress homologous recombination through JAK2/STAT4/BARD1 axis in human NSCLC cells.

Authors - Jian Zhang
View Article