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RNF31 / HOIP Recombinant antibody

RNF31 / HOIP Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230446E9

Cat no : 83137-4-RR

Print datasheet

Synonyms

HOIP, RNF31, HOIL 1 interacting protein, FLJ23501, FLJ10111

验证数据展示

  • WB analysis using 83137-4-RR

    WB analysis using 83137-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83137-4-RR (RNF31 / HOIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of PC-3 using 83137-4-RR

    FC experiment of PC-3 using 83137-4-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.2 ug RNF31 / HOIP Recombinant antibody (83137-4-RR, Clone:230446E9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, HepG2 cells
Positive FC (Intra) detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83137-4-RR is RNF31 / HOIP Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen RNF31 / HOIP fusion protein Ag34505 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ring finger protein 31
Synonyms HOIP, RNF31, HOIL 1 interacting protein, FLJ23501, FLJ10111
Calculated Molecular Weight 1072 aa, 120 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC017376
Gene Symbol RNF31
Gene ID (NCBI) 55072
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96EP0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RNF31 / HOIP antibody 83137-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols