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RNF34 Polyclonal antibody

RNF34 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 10629-1-AP

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Synonyms

CARP 1, Caspase regulator CARP1, FYVE RING finger protein Momo, hRFI, RFI, RIF, RIFF, ring finger protein 34, RING finger protein RIFF, RNF34



经过测试的应用

Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10629-1-AP targets RNF34 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen RNF34 fusion protein Ag0982 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 34
Synonyms CARP 1, Caspase regulator CARP1, FYVE RING finger protein Momo, hRFI, RFI, RIF, RIFF, ring finger protein 34, RING finger protein RIFF, RNF34
Calculated Molecular Weight 42 kDa
GenBank Accession NumberBC007826
Gene Symbol RNF34
Gene ID (NCBI) 80196
RRIDAB_2301135
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ969K3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF34 is an E3 ubiquitin ligase that regulates protein substrates by post-translational modification. It is associated with regulating postsynaptic γ2-GABAAR clustering and GABAergic synaptic innervation in hippocampal neurons, indicating the importance of RNF34 in regulating neurological function.It is preferentially expressed in esophageal, gastric, and colorectal cancers, suggesting a possible association between RNF34 and the development of digestive tract cancers. Immunoblots showed a single 42-kDa protein band corresponding to the molecular weight of RNF34.(PMID: 31704983, PMID: 25193658)

实验方案

Product Specific Protocols
IHC protocol for RNF34 antibody 10629-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF

Nat Commun

A ZFYVE21-Rubicon-RNF34 signaling complex promotes endosome-associated inflammasome activity in endothelial cells

Authors - Xue Li
  • KD Validated