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RNF4 Polyclonal antibody

RNF4 Polyclonal Antibody for WB, IP, ELISA
Cat No. 17810-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA and More (1)

RES4-26, RES4 26, Protein SNURF, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inPC-3 cells
Positive IP detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy RNF4 Ready-To-Use IHC Kit. RNF4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17810-1-AP targets RNF4 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 RNF4 fusion protein Ag12115 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 4
别名 RES4-26, RES4 26, Protein SNURF, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF4
计算分子量 190 aa, 21 kDa
观测分子量 30 kDa
GenBank蛋白编号BC031935
基因名称 RNF4
Gene ID (NCBI) 6047
RRIDAB_2878443
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP78317
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF4 is a E3 ubiquitin-protein ligase that binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. It regulates the degradation of several proteins including PML and PEA3[PMID:18408734]. It also participates in chromosome alignment and spindle assembly, and regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. In addition, RNF4 can regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 [PMID:20026589]. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation [PMID:11319220]. RNF4 exist as two isoforms. Isoform one is a 30 kDa protein and isoform two is a about 15 kDa protein. RNF4 binds polysumoylated chains and the molecular weight of RNF4 migrate in the experiment to 35 kDa, 47 kDa, 69 kDa as ubiquitinated protein.

实验方案

Product Specific Protocols
WB protocol for RNF4 antibody 17810-1-APDownload protocol
IP protocol for RNF4 antibody 17810-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival

Authors - Pauline Lascaux
humanWB

Nat Commun

Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin

Authors - Tao Zhang
humanWB

Int J Mol Sci

The E3 Ubiquitin-Protein Ligase RNF4 Promotes TNF-α-Induced Cell Death Triggered by RIPK1.

Authors - Tatsuya Shimada
humanWB

ACS Chem Biol

Covalent Ligand Screening Uncovers a RNF4 E3 Ligase Recruiter for Targeted Protein Degradation Applications.

Authors - Carl C Ward
humanWB

J Biol Chem

Analysis of a degron-containing reporter protein GFP-CL1 reveals a role for SUMO1 in cytosolic protein quality control

Authors - Wei Wang
  • KD Validated
mouseWB,CoIP

Theranostics

CSF2RB overexpression promotes the protective effects of mesenchymal stromal cells against ischemic heart injury

Authors - Tingting Qi
  • KD Validated
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