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验证数据展示

  • WB analysis of HEK-293 using 15621-1-AP

    WB analysis of HEK-293 using 15621-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15621-1-AP (RNF40 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 15621-1-AP

    WB analysis of mouse liver using 15621-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15621-1-AP (RNF40 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15621-1-AP

    IF Staining of HeLa using 15621-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15621-1-AP (RNF40 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

RNF40 Polyclonal antibody

RNF40 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 15621-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

EC:2.3.2.27, E3 ubiquitin-protein ligase BRE1B, BRE1-B, BRE1B, BRE1 B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse liver tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15621-1-AP targets RNF40 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen RNF40 fusion protein Ag8047 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 40
Synonyms EC:2.3.2.27, E3 ubiquitin-protein ligase BRE1B, BRE1-B, BRE1B, BRE1 B
Calculated Molecular Weight 114 kDa
Observed Molecular Weight110-140 kDa
GenBank Accession NumberBC006133
Gene Symbol RNF40
Gene ID (NCBI) 9810
RRIDAB_2253949
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75150
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The DNA damage response (DDR) is emerging as a vast signaling network that temporarily modulates numerous aspects of cellular metabolism in the face of DNA lesions, especially critical ones such as the double-strand break (DSB). The DDR involves extensive dynamics of protein post-translational modifications, most notably phosphorylation and ubiquitylation. A recently identified novel component of the DDR pathway - histone H2B monoubiquitylation - exemplifies this principle. Monoubiquitination of histone H2B is a dynamic post-translational histone modification associated with transcriptional elongation and the DNA damage response [PMID:22021426]. In mammalian cells, H2B monoubiquitylation is driven primarily by an E3 ubiquitin ligase composed of the two RING finger proteins RNF20 and RNF40 [PMID:22031019,21827756].

实验方案

Product Specific Protocols
WB protocol for RNF40 antibody 15621-1-APDownload protocol
IF protocol for RNF40 antibody 15621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols